Haemogenic endocardium contributes to transient definitive haematopoiesis

Haruko Nakano, Xiaoqian Liu, Armin Arshi, Yasuhiro Nakashima, Ben Van Handel, Rajkumar Sasidharan, Andrew W. Harmon, Jae Ho Shin, Robert J. Schwartz, Simon J. Conway, Richard P. Harvey, Mohammad Pashmforoush, Hanna K.A. Mikkola, Atsushi Nakano

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Abstract

Haematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial and haematopoietic lineages, it remains unknown whether the mammalian heart tube serves as a haemogenic organ akin to the dorsal aorta. Here we examine the haemogenic activity of the developing endocardium. Mouse heart explants generate myeloid and erythroid colonies in the absence of circulation. Haemogenic activity arises from a subset of endocardial cells in the outflow cushion and atria earlier than in the aorta-gonad-mesonephros region, and is transient and definitive in nature. Interestingly, key cardiac transcription factors, Nkx2-5 and Isl1, are expressed in and required for the haemogenic population of the endocardium. Together, these data suggest that a subset of endocardial/endothelial cells serve as a de novo source for transient definitive haematopoietic progenitors.

Original languageEnglish (US)
Article number1564
JournalNature communications
Volume4
DOIs
StatePublished - Apr 11 2013

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ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Nakano, H., Liu, X., Arshi, A., Nakashima, Y., Van Handel, B., Sasidharan, R., Harmon, A. W., Shin, J. H., Schwartz, R. J., Conway, S. J., Harvey, R. P., Pashmforoush, M., Mikkola, H. K. A., & Nakano, A. (2013). Haemogenic endocardium contributes to transient definitive haematopoiesis. Nature communications, 4, [1564]. https://doi.org/10.1038/ncomms2569