Haemogenic endocardium contributes to transient definitive haematopoiesis

Haruko Nakano, Xiaoqian Liu, Armin Arshi, Yasuhiro Nakashima, Ben Van Handel, Rajkumar Sasidharan, Andrew W. Harmon, Jae Ho Shin, Robert J. Schwartz, Simon Conway, Richard P. Harvey, Mohammad Pashmforoush, Hanna K A Mikkola, Atsushi Nakano

Research output: Contribution to journalArticle

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Abstract

Haematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial and haematopoietic lineages, it remains unknown whether the mammalian heart tube serves as a haemogenic organ akin to the dorsal aorta. Here we examine the haemogenic activity of the developing endocardium. Mouse heart explants generate myeloid and erythroid colonies in the absence of circulation. Haemogenic activity arises from a subset of endocardial cells in the outflow cushion and atria earlier than in the aorta-gonad-mesonephros region, and is transient and definitive in nature. Interestingly, key cardiac transcription factors, Nkx2-5 and Isl1, are expressed in and required for the haemogenic population of the endocardium. Together, these data suggest that a subset of endocardial/endothelial cells serve as a de novo source for transient definitive haematopoietic progenitors.

Original languageEnglish
Article number1564
JournalNature Communications
Volume4
DOIs
StatePublished - 2013

Fingerprint

hematopoiesis
Endocardium
aorta
Hematopoiesis
set theory
Aorta
gonads
Mesonephros
cushions
stem cells
embryos
Endothelial cells
Gonads
Embryonic Stem Cells
Stem cells
cells
organs
mice
animals
Animals

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Nakano, H., Liu, X., Arshi, A., Nakashima, Y., Van Handel, B., Sasidharan, R., ... Nakano, A. (2013). Haemogenic endocardium contributes to transient definitive haematopoiesis. Nature Communications, 4, [1564]. https://doi.org/10.1038/ncomms2569

Haemogenic endocardium contributes to transient definitive haematopoiesis. / Nakano, Haruko; Liu, Xiaoqian; Arshi, Armin; Nakashima, Yasuhiro; Van Handel, Ben; Sasidharan, Rajkumar; Harmon, Andrew W.; Shin, Jae Ho; Schwartz, Robert J.; Conway, Simon; Harvey, Richard P.; Pashmforoush, Mohammad; Mikkola, Hanna K A; Nakano, Atsushi.

In: Nature Communications, Vol. 4, 1564, 2013.

Research output: Contribution to journalArticle

Nakano, H, Liu, X, Arshi, A, Nakashima, Y, Van Handel, B, Sasidharan, R, Harmon, AW, Shin, JH, Schwartz, RJ, Conway, S, Harvey, RP, Pashmforoush, M, Mikkola, HKA & Nakano, A 2013, 'Haemogenic endocardium contributes to transient definitive haematopoiesis', Nature Communications, vol. 4, 1564. https://doi.org/10.1038/ncomms2569
Nakano H, Liu X, Arshi A, Nakashima Y, Van Handel B, Sasidharan R et al. Haemogenic endocardium contributes to transient definitive haematopoiesis. Nature Communications. 2013;4. 1564. https://doi.org/10.1038/ncomms2569
Nakano, Haruko ; Liu, Xiaoqian ; Arshi, Armin ; Nakashima, Yasuhiro ; Van Handel, Ben ; Sasidharan, Rajkumar ; Harmon, Andrew W. ; Shin, Jae Ho ; Schwartz, Robert J. ; Conway, Simon ; Harvey, Richard P. ; Pashmforoush, Mohammad ; Mikkola, Hanna K A ; Nakano, Atsushi. / Haemogenic endocardium contributes to transient definitive haematopoiesis. In: Nature Communications. 2013 ; Vol. 4.
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