Haemophilus ducreyi adheres to human keratinocytes

Renier J. Brentjens, Stanley Spinola, Anthony A. Campagnari

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Haemophilus ducreyi, Moraxella catarrhalis and a non-piliated Escherichia coli K-12 strain were studied for their ability to bind to human keratinocytes in vitro. Epidermal cells isolated from neonatal foreskins were grown to confluence in serum-free keratinocyte media. Probing of the monolayers with anti-cytokeratin antibody showed that 97% of cells were keratinocytes. Bacteria were grown to mid-log phase and seeded onto the monolayers. At various timepoints monolayers were washed with PBS to remove non-adherent bacteria, and the monolayers were quantitatively cultured. After 120 min, 15 to 23% of the H. ducreyi inocula bound to the monolayer, while less than 1% of the M. catarrhalis or E. coli controls bound. Wet mounts of fixed monolayers observed with differential interference contrast microscopy confirmed the quantitative data. We conclude that H. ducreyi binds to keratinocytes and that this process may play a role in the initiation of chancroid.

Original languageEnglish (US)
Pages (from-to)243-247
Number of pages5
JournalMicrobial Pathogenesis
Volume16
Issue number3
DOIs
StatePublished - Mar 1994

Fingerprint

Haemophilus ducreyi
Keratinocytes
Moraxella (Branhamella) catarrhalis
Chancroid
Interference Microscopy
Escherichia coli
Bacteria
Foreskin
Serum-Free Culture Media
Keratins
Anti-Idiotypic Antibodies

Keywords

  • Haemophilus ducreyi; adherence; keratinocytes

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology

Cite this

Haemophilus ducreyi adheres to human keratinocytes. / Brentjens, Renier J.; Spinola, Stanley; Campagnari, Anthony A.

In: Microbial Pathogenesis, Vol. 16, No. 3, 03.1994, p. 243-247.

Research output: Contribution to journalArticle

Brentjens, Renier J. ; Spinola, Stanley ; Campagnari, Anthony A. / Haemophilus ducreyi adheres to human keratinocytes. In: Microbial Pathogenesis. 1994 ; Vol. 16, No. 3. pp. 243-247.
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