Haemophilus ducreyi elicits a cutaneous infiltrate of CD4 cells during experimental human infection

Stanley M. Spinola, Attilio Orazi, Janet N. Arno, Kate Fortney, Patricia Kotylo, Cheng Yen Chen, Anthony A. Campagnari, Antoinette F. Hood

Research output: Contribution to journalArticle

105 Scopus citations

Abstract

Human subjects were experimentally infected with Haemophilus ducreyi for up to 2 weeks. Bacterial suspensions were delivered into the epidermis and dermis through puncture wounds made by an allergy-testing device. Subjects developed papular lesions that evolved into pustules resembling natural disease. Some papular lesions resolved spontaneously, indicating that host responses may clear infection. Bacteria were shed intermittently from lesions, suggesting that H. ducreyi may be transmissible before ulceration. Host responses to infection consisted primarily of cutaneous infiltrate of polymorphonuclear leukocytes, Langerhans cells, macrophages, and CD4 T cells of αβ lineage. Expression of HLA-DR by keratinocytes was associated with the presence of interferon-γ mRNA in the skin. There was little evidence for humoral or peripheral blood mononuclear cell responses to bacterial antigens. The cutaneous infiltrate of CD4 cells and macrophages provides a mechanism that facilitates transmission of human immunodeficiency virus by H. ducreyi.

Original languageEnglish (US)
Pages (from-to)394-402
Number of pages9
JournalJournal of Infectious Diseases
Volume173
Issue number2
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Spinola, S. M., Orazi, A., Arno, J. N., Fortney, K., Kotylo, P., Chen, C. Y., Campagnari, A. A., & Hood, A. F. (1996). Haemophilus ducreyi elicits a cutaneous infiltrate of CD4 cells during experimental human infection. Journal of Infectious Diseases, 173(2), 394-402. https://doi.org/10.1093/infdis/173.2.394