Haemophilus ducreyi elicits a cutaneous infiltrate of CD4 cells during experimental human infection

Stanley Spinola, Attilio Orazi, Janet Arno, Kate Fortney, Patricia Kotylo, Cheng Yen Chen, Anthony A. Campagnari, Antoinette F. Hood

Research output: Contribution to journalArticle

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Abstract

Human subjects were experimentally infected with Haemophilus ducreyi for up to 2 weeks. Bacterial suspensions were delivered into the epidermis and dermis through puncture wounds made by an allergy-testing device. Subjects developed papular lesions that evolved into pustules resembling natural disease. Some papular lesions resolved spontaneously, indicating that host responses may clear infection. Bacteria were shed intermittently from lesions, suggesting that H. ducreyi may be transmissible before ulceration. Host responses to infection consisted primarily of cutaneous infiltrate of polymorphonuclear leukocytes, Langerhans cells, macrophages, and CD4 T cells of αβ lineage. Expression of HLA-DR by keratinocytes was associated with the presence of interferon-γ mRNA in the skin. There was little evidence for humoral or peripheral blood mononuclear cell responses to bacterial antigens. The cutaneous infiltrate of CD4 cells and macrophages provides a mechanism that facilitates transmission of human immunodeficiency virus by H. ducreyi.

Original languageEnglish
Pages (from-to)394-402
Number of pages9
JournalJournal of Infectious Diseases
Volume173
Issue number2
StatePublished - 1996

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Haemophilus ducreyi
Skin
Infection
Macrophages
Bacterial Antigens
Langerhans Cells
HLA-DR Antigens
Cell Lineage
Dermis
Keratinocytes
Punctures
Epidermis
Interferons
Blood Cells
Suspensions
Hypersensitivity
Neutrophils
HIV
Bacteria
T-Lymphocytes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Spinola, S., Orazi, A., Arno, J., Fortney, K., Kotylo, P., Chen, C. Y., ... Hood, A. F. (1996). Haemophilus ducreyi elicits a cutaneous infiltrate of CD4 cells during experimental human infection. Journal of Infectious Diseases, 173(2), 394-402.

Haemophilus ducreyi elicits a cutaneous infiltrate of CD4 cells during experimental human infection. / Spinola, Stanley; Orazi, Attilio; Arno, Janet; Fortney, Kate; Kotylo, Patricia; Chen, Cheng Yen; Campagnari, Anthony A.; Hood, Antoinette F.

In: Journal of Infectious Diseases, Vol. 173, No. 2, 1996, p. 394-402.

Research output: Contribution to journalArticle

Spinola, S, Orazi, A, Arno, J, Fortney, K, Kotylo, P, Chen, CY, Campagnari, AA & Hood, AF 1996, 'Haemophilus ducreyi elicits a cutaneous infiltrate of CD4 cells during experimental human infection', Journal of Infectious Diseases, vol. 173, no. 2, pp. 394-402.
Spinola, Stanley ; Orazi, Attilio ; Arno, Janet ; Fortney, Kate ; Kotylo, Patricia ; Chen, Cheng Yen ; Campagnari, Anthony A. ; Hood, Antoinette F. / Haemophilus ducreyi elicits a cutaneous infiltrate of CD4 cells during experimental human infection. In: Journal of Infectious Diseases. 1996 ; Vol. 173, No. 2. pp. 394-402.
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