Haemophilus ducreyi lipooligosaccharide mutant defective in expression of β-1,4-glucosyltransferase is virulent in humans

R. S. Young, M. J. Filiatrault, K. R. Fortney, A. F. Hood, Barry Katz, Jr Munson R.S., A. A. Campagnari, Stanley Spinola

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The lipooligosaccharide (LOS) of Haemophilus ducreyi contains a major glycoform that is immunochemically identical to paragloboside, a glycosphingolipid precursor of major human blood group antigens. We recently identified the gene responsible for the glucosyltransferase activity and constructed an isogenic mutant (35000glu-) deficient in this activity. 35000glu- makes an LOS that consists only of the heptose trisaccharide core and 2-keto-deoxyoctulosonic acid (KDO). For this study, the mutant was reconstructed in the 35000HP (human passaged [HP]) background. Five human subjects were inoculated with 35000HP and 35000HPglu- in a dose-response trial. The pustule formation rates were 40% (95% confidence interval [CI], 13.7 to 72.6%) at 10 sites for 35000HP and 46.7% (95% CI, 24.8 to 69.9%) at 15 sites for 35000HPglu-. The histopathology and recovery rates of H. ducreyi from surface cultures and biopsies obtained from mutant and parent sites were similar. These results indicate that the expression of glycoforms with sugar moieties extending beyond the heptose trisaccharide core is not required for pustule formation by H. ducreyi in humans.

Original languageEnglish
Pages (from-to)4180-4184
Number of pages5
JournalInfection and Immunity
Volume69
Issue number6
DOIs
StatePublished - 2001

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Haemophilus ducreyi
Glucosyltransferases
Heptoses
Trisaccharides
Confidence Intervals
Keto Acids
Glycosphingolipids
Blood Group Antigens
Biopsy
lipid-linked oligosaccharides
Genes

ASJC Scopus subject areas

  • Immunology

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Haemophilus ducreyi lipooligosaccharide mutant defective in expression of β-1,4-glucosyltransferase is virulent in humans. / Young, R. S.; Filiatrault, M. J.; Fortney, K. R.; Hood, A. F.; Katz, Barry; Munson R.S., Jr; Campagnari, A. A.; Spinola, Stanley.

In: Infection and Immunity, Vol. 69, No. 6, 2001, p. 4180-4184.

Research output: Contribution to journalArticle

Young, R. S. ; Filiatrault, M. J. ; Fortney, K. R. ; Hood, A. F. ; Katz, Barry ; Munson R.S., Jr ; Campagnari, A. A. ; Spinola, Stanley. / Haemophilus ducreyi lipooligosaccharide mutant defective in expression of β-1,4-glucosyltransferase is virulent in humans. In: Infection and Immunity. 2001 ; Vol. 69, No. 6. pp. 4180-4184.
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