Ham-2 corrects the class I antigen-processing defect in RMA-S cells

Michelle Attaya, Stephen Jameson, Coleen K. Martinez, Evan Hermel, Carla Aldrich, James Forman, Kirsten Fischer Lindahl, Michael J. Bevan, John J. Monaco

Research output: Contribution to journalArticle

254 Citations (Scopus)

Abstract

THE murine major histocompatibility complex (MHC) contains two genes (Ham-1 and Ham-2) that encode members of a superfamily of ATP-dependent transport proteins1,2. These genes are believed to mediate the transport of peptide antigen from the cytoplasm into the lumen of the endoplasmic reticulum for binding by MHC class I molecules. Evidence for such a function has come from the rescue of class I surface expression by a cloned copy of the human homologue of Ham-1, PSF-1, in a human cell line that is defective in antigen processing3. A mutant murine cell line, RMA-S, has an identical antigen-processing-defective phenotype4,5. Here we show that expression of a cloned copy of the Ham-2 gene in RMA-S cells results in recovery of the ability to process and present class I-restricted antigens to cytotoxic T lymphocytes, and in partial recovery of class I surface expression. Processing defects for classical (H-2 K and D) and non-classical (Qa1 and HMT) class I molecules are corrected by Ham-2. These data indicate that both MHC-linked transporter genes are probably required for class I antigen processing, and that the functional transporter in this pathway may consist of a Ham-1/Ham-2 heterodimer.

Original languageEnglish (US)
Pages (from-to)647-649
Number of pages3
JournalNature
Volume355
Issue number6361
StatePublished - Feb 13 1992
Externally publishedYes

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Histocompatibility Antigens Class I
Antigen Presentation
Major Histocompatibility Complex
Genes
Antigens
Cell Line
Cytotoxic T-Lymphocytes
Endoplasmic Reticulum
Cytoplasm
Adenosine Triphosphate
Peptides

ASJC Scopus subject areas

  • General

Cite this

Attaya, M., Jameson, S., Martinez, C. K., Hermel, E., Aldrich, C., Forman, J., ... Monaco, J. J. (1992). Ham-2 corrects the class I antigen-processing defect in RMA-S cells. Nature, 355(6361), 647-649.

Ham-2 corrects the class I antigen-processing defect in RMA-S cells. / Attaya, Michelle; Jameson, Stephen; Martinez, Coleen K.; Hermel, Evan; Aldrich, Carla; Forman, James; Lindahl, Kirsten Fischer; Bevan, Michael J.; Monaco, John J.

In: Nature, Vol. 355, No. 6361, 13.02.1992, p. 647-649.

Research output: Contribution to journalArticle

Attaya, M, Jameson, S, Martinez, CK, Hermel, E, Aldrich, C, Forman, J, Lindahl, KF, Bevan, MJ & Monaco, JJ 1992, 'Ham-2 corrects the class I antigen-processing defect in RMA-S cells', Nature, vol. 355, no. 6361, pp. 647-649.
Attaya M, Jameson S, Martinez CK, Hermel E, Aldrich C, Forman J et al. Ham-2 corrects the class I antigen-processing defect in RMA-S cells. Nature. 1992 Feb 13;355(6361):647-649.
Attaya, Michelle ; Jameson, Stephen ; Martinez, Coleen K. ; Hermel, Evan ; Aldrich, Carla ; Forman, James ; Lindahl, Kirsten Fischer ; Bevan, Michael J. ; Monaco, John J. / Ham-2 corrects the class I antigen-processing defect in RMA-S cells. In: Nature. 1992 ; Vol. 355, No. 6361. pp. 647-649.
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