Head Module Control of Mediator Interactions

Yuichiro Takagi, Guillermo Calero, Hirofumi Komori, Jesse A. Brown, Andreas H. Ehrensberger, Andy Hudmon, Francisco Asturias, Roger D. Kornberg

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Yeast Mediator proteins interacting with Med17(Srb4) have been expressed at a high level with the use of recombinant baculoviruses and recovered in homogeneous form as a seven subunit, 223 kDa complex. Electron microscopy and single-particle analysis identify this complex as the Mediator head module. The recombinant head module complements "headless" Mediator for the initiation of transcription in vitro. The module interacts with an RNA polymerase II-TFIIF complex, but not with the polymerase or TFIIF alone. This interaction is lost in the presence of a DNA template and associated RNA transcript, recapitulating the release of Mediator that occurs upon the initiation of transcription. Disruption of the head module in a temperature-sensitive mutant in vivo leads to the release of middle and tail modules from a transcriptionally active promoter. The head module evidently controls Mediator-RNA polymerase II and Mediator-promoter interactions.

Original languageEnglish (US)
Pages (from-to)355-364
Number of pages10
JournalMolecular Cell
Volume23
Issue number3
DOIs
StatePublished - Aug 4 2006
Externally publishedYes

Fingerprint

RNA Polymerase II
Head
Mediator Complex
Fungal Proteins
Baculoviridae
Electron Microscopy
RNA
Temperature
DNA
transcription factor TFIIF
In Vitro Techniques

Keywords

  • DNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Takagi, Y., Calero, G., Komori, H., Brown, J. A., Ehrensberger, A. H., Hudmon, A., ... Kornberg, R. D. (2006). Head Module Control of Mediator Interactions. Molecular Cell, 23(3), 355-364. https://doi.org/10.1016/j.molcel.2006.06.007

Head Module Control of Mediator Interactions. / Takagi, Yuichiro; Calero, Guillermo; Komori, Hirofumi; Brown, Jesse A.; Ehrensberger, Andreas H.; Hudmon, Andy; Asturias, Francisco; Kornberg, Roger D.

In: Molecular Cell, Vol. 23, No. 3, 04.08.2006, p. 355-364.

Research output: Contribution to journalArticle

Takagi, Y, Calero, G, Komori, H, Brown, JA, Ehrensberger, AH, Hudmon, A, Asturias, F & Kornberg, RD 2006, 'Head Module Control of Mediator Interactions', Molecular Cell, vol. 23, no. 3, pp. 355-364. https://doi.org/10.1016/j.molcel.2006.06.007
Takagi Y, Calero G, Komori H, Brown JA, Ehrensberger AH, Hudmon A et al. Head Module Control of Mediator Interactions. Molecular Cell. 2006 Aug 4;23(3):355-364. https://doi.org/10.1016/j.molcel.2006.06.007
Takagi, Yuichiro ; Calero, Guillermo ; Komori, Hirofumi ; Brown, Jesse A. ; Ehrensberger, Andreas H. ; Hudmon, Andy ; Asturias, Francisco ; Kornberg, Roger D. / Head Module Control of Mediator Interactions. In: Molecular Cell. 2006 ; Vol. 23, No. 3. pp. 355-364.
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