Healing of critical-size segmental defects in rat femora using strong porous bioactive glass scaffolds

Lianxiang Bi, Brett Zobell, Xin Liu, Mohamed N. Rahaman, Lynda F. Bonewald

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The repair of structural bone defects such as segmental defects in the long bones of the limbs is a challenging clinical problem. In this study, the capacity of silicate (13-93) and borate (13-93B3) bioactive glass scaffolds (porosity = 47-50%) to heal critical-size segmental defects in rat femurs was evaluated and compared with autografts. Defects were implanted with 13-93 and 13-93B3 scaffolds with a grid-like microstructure (compressive strength = 86 MPa and 40 MPa, respectively), 13-93B3 scaffolds with an oriented microstructure (compressive strength = 32 MPa) and autografts using intramedullary fixation. Twelve weeks post-implantation, the defects were harvested and evaluated using histomorphometric analysis. The percentage of new bone in the defects implanted with the three groups of glass scaffolds (25-28%) and the total von Kossa-positive area (32-38%) were not significantly different from the autografts (new bone = 38%; von Kossa-positive area = 40%) (p > 0.05). New blood vessel area in the defects implanted with the glass scaffolds (4-8%) and the autografts (5%) showed no significant difference among the four groups. New cartilage formed in the 13-93 grid-like scaffolds (18%) was significantly higher than in 13-93B3 grid-like scaffolds (8%) and in the autografts (8%) (p = 0.02). The results indicate that these strong porous bioactive glass scaffolds are promising synthetic implants for structural bone repair.

Original languageEnglish (US)
Pages (from-to)816-824
Number of pages9
JournalMaterials Science and Engineering C
Volume42
DOIs
StatePublished - Sep 1 2014

Fingerprint

femur
Bioactive glass
Autografts
healing
activity (biology)
Scaffolds
Femur
rats
Glass
Rats
bones
Bone and Bones
Defects
glass
defects
Compressive Strength
Bone
compressive strength
grids
Silicates

Keywords

  • Bioactive glass scaffold
  • Bone regeneration
  • Histomorphometric analysis
  • Rodent model
  • Segmental bone defects

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Healing of critical-size segmental defects in rat femora using strong porous bioactive glass scaffolds. / Bi, Lianxiang; Zobell, Brett; Liu, Xin; Rahaman, Mohamed N.; Bonewald, Lynda F.

In: Materials Science and Engineering C, Vol. 42, 01.09.2014, p. 816-824.

Research output: Contribution to journalArticle

@article{fdae197c902c45be9aa62ab424d05b83,
title = "Healing of critical-size segmental defects in rat femora using strong porous bioactive glass scaffolds",
abstract = "The repair of structural bone defects such as segmental defects in the long bones of the limbs is a challenging clinical problem. In this study, the capacity of silicate (13-93) and borate (13-93B3) bioactive glass scaffolds (porosity = 47-50{\%}) to heal critical-size segmental defects in rat femurs was evaluated and compared with autografts. Defects were implanted with 13-93 and 13-93B3 scaffolds with a grid-like microstructure (compressive strength = 86 MPa and 40 MPa, respectively), 13-93B3 scaffolds with an oriented microstructure (compressive strength = 32 MPa) and autografts using intramedullary fixation. Twelve weeks post-implantation, the defects were harvested and evaluated using histomorphometric analysis. The percentage of new bone in the defects implanted with the three groups of glass scaffolds (25-28{\%}) and the total von Kossa-positive area (32-38{\%}) were not significantly different from the autografts (new bone = 38{\%}; von Kossa-positive area = 40{\%}) (p > 0.05). New blood vessel area in the defects implanted with the glass scaffolds (4-8{\%}) and the autografts (5{\%}) showed no significant difference among the four groups. New cartilage formed in the 13-93 grid-like scaffolds (18{\%}) was significantly higher than in 13-93B3 grid-like scaffolds (8{\%}) and in the autografts (8{\%}) (p = 0.02). The results indicate that these strong porous bioactive glass scaffolds are promising synthetic implants for structural bone repair.",
keywords = "Bioactive glass scaffold, Bone regeneration, Histomorphometric analysis, Rodent model, Segmental bone defects",
author = "Lianxiang Bi and Brett Zobell and Xin Liu and Rahaman, {Mohamed N.} and Bonewald, {Lynda F.}",
year = "2014",
month = "9",
day = "1",
doi = "10.1016/j.msec.2014.06.022",
language = "English (US)",
volume = "42",
pages = "816--824",
journal = "Materials Science and Engineering C",
issn = "0928-4931",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Healing of critical-size segmental defects in rat femora using strong porous bioactive glass scaffolds

AU - Bi, Lianxiang

AU - Zobell, Brett

AU - Liu, Xin

AU - Rahaman, Mohamed N.

AU - Bonewald, Lynda F.

PY - 2014/9/1

Y1 - 2014/9/1

N2 - The repair of structural bone defects such as segmental defects in the long bones of the limbs is a challenging clinical problem. In this study, the capacity of silicate (13-93) and borate (13-93B3) bioactive glass scaffolds (porosity = 47-50%) to heal critical-size segmental defects in rat femurs was evaluated and compared with autografts. Defects were implanted with 13-93 and 13-93B3 scaffolds with a grid-like microstructure (compressive strength = 86 MPa and 40 MPa, respectively), 13-93B3 scaffolds with an oriented microstructure (compressive strength = 32 MPa) and autografts using intramedullary fixation. Twelve weeks post-implantation, the defects were harvested and evaluated using histomorphometric analysis. The percentage of new bone in the defects implanted with the three groups of glass scaffolds (25-28%) and the total von Kossa-positive area (32-38%) were not significantly different from the autografts (new bone = 38%; von Kossa-positive area = 40%) (p > 0.05). New blood vessel area in the defects implanted with the glass scaffolds (4-8%) and the autografts (5%) showed no significant difference among the four groups. New cartilage formed in the 13-93 grid-like scaffolds (18%) was significantly higher than in 13-93B3 grid-like scaffolds (8%) and in the autografts (8%) (p = 0.02). The results indicate that these strong porous bioactive glass scaffolds are promising synthetic implants for structural bone repair.

AB - The repair of structural bone defects such as segmental defects in the long bones of the limbs is a challenging clinical problem. In this study, the capacity of silicate (13-93) and borate (13-93B3) bioactive glass scaffolds (porosity = 47-50%) to heal critical-size segmental defects in rat femurs was evaluated and compared with autografts. Defects were implanted with 13-93 and 13-93B3 scaffolds with a grid-like microstructure (compressive strength = 86 MPa and 40 MPa, respectively), 13-93B3 scaffolds with an oriented microstructure (compressive strength = 32 MPa) and autografts using intramedullary fixation. Twelve weeks post-implantation, the defects were harvested and evaluated using histomorphometric analysis. The percentage of new bone in the defects implanted with the three groups of glass scaffolds (25-28%) and the total von Kossa-positive area (32-38%) were not significantly different from the autografts (new bone = 38%; von Kossa-positive area = 40%) (p > 0.05). New blood vessel area in the defects implanted with the glass scaffolds (4-8%) and the autografts (5%) showed no significant difference among the four groups. New cartilage formed in the 13-93 grid-like scaffolds (18%) was significantly higher than in 13-93B3 grid-like scaffolds (8%) and in the autografts (8%) (p = 0.02). The results indicate that these strong porous bioactive glass scaffolds are promising synthetic implants for structural bone repair.

KW - Bioactive glass scaffold

KW - Bone regeneration

KW - Histomorphometric analysis

KW - Rodent model

KW - Segmental bone defects

UR - http://www.scopus.com/inward/record.url?scp=84904540792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904540792&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2014.06.022

DO - 10.1016/j.msec.2014.06.022

M3 - Article

C2 - 25063184

AN - SCOPUS:84904540792

VL - 42

SP - 816

EP - 824

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

ER -