Heart-type fatty acid-binding protein reciprocally regulates glucose and fatty acid utilization during exercise

Jane Shearer, Patrick T. Fueger, Jeffrey N. Rottman, Deanna P. Bracy, Bert Binas, David H. Wasserman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The role of heart-type cytosolic fatty acid-binding protein (H-FABP) in mediating whole body and muscle-specific long-chain fatty acid (LCFA) and glucose utilization was examined using exercise as a phenotyping tool. Catheters were chronically implanted in a carotid artery and jugular vein of wild-type (WT, n = 8), heterozygous (H-FABP+/-, n = 8), and null (H-FABP -/-, n = 7) chow-fed C57BL/6J mice, and mice were allowed to recover for 7 days. After a 5-h fast, conscious, unrestrained mice were studied during 30 min of treadmill exercise (0.6 mph). A bolus of [125I]-15-(p- iodophenyl)-3-R,S-methylpentadecanoic acid and 2-deoxy-[3H]glucose was administered to obtain rates of whole body metabolic clearance (MCR) and indexes of muscle LCFA (Rf) and glucose (Rg) utilization. Fasting, nonesterified fatty acids (mM) were elevated in H-FABP-/- mice (2.2 ± 0.9 vs. 1.3 ± 0.1 and 1.3 ± 0.2 for WT and H-FABP+/-). During exercise, blood glucose (mM) increased in WT (11.7 ± 0.8) and H-FABP+/- (12.6 ± 0.9) mice, whereas H-FABP-/- mice developed overt hypoglycemia (4.8 ± 0.8). Examination of tissue-specific and whole body glucose and LCFA utilization demonstrated a dependency on H-FABP with exercise in all tissues examined. Reductions in H-FABP led to decreasing exercise-stimulated Rf and increasing Rg with the most pronounced effects in heart and soleus muscle. Similar results were seen for MCR with decreasing LCFA and increasing glucose clearance with declining levels of H-FABP. These results show that, in vivo, H-FABP has reciprocal effects on glucose and LCFA utilization and whole body fuel homeostasis when metabolic demands are elevated by exercise.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume288
Issue number2 51-2
DOIs
StatePublished - Feb 2005
Externally publishedYes

Fingerprint

Fatty Acid-Binding Proteins
Fatty Acids
Glucose
Muscle
Tissue
Muscles
Exercise equipment
Jugular Veins
Catheters
Inbred C57BL Mouse
Carotid Arteries
Hypoglycemia
Nonesterified Fatty Acids
Blood Glucose
Fasting
Myocardium
Skeletal Muscle
Homeostasis

Keywords

  • 15-(p-iodophenyl)-3-R,S-methylpentadecanoic acid
  • 2-deoxyglucose
  • Metabolism
  • Skeletal muscle
  • Substrate balance

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Heart-type fatty acid-binding protein reciprocally regulates glucose and fatty acid utilization during exercise. / Shearer, Jane; Fueger, Patrick T.; Rottman, Jeffrey N.; Bracy, Deanna P.; Binas, Bert; Wasserman, David H.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 288, No. 2 51-2, 02.2005.

Research output: Contribution to journalArticle

Shearer, Jane ; Fueger, Patrick T. ; Rottman, Jeffrey N. ; Bracy, Deanna P. ; Binas, Bert ; Wasserman, David H. / Heart-type fatty acid-binding protein reciprocally regulates glucose and fatty acid utilization during exercise. In: American Journal of Physiology - Endocrinology and Metabolism. 2005 ; Vol. 288, No. 2 51-2.
@article{4a13c98853064e879056c71ccd91b7fb,
title = "Heart-type fatty acid-binding protein reciprocally regulates glucose and fatty acid utilization during exercise",
abstract = "The role of heart-type cytosolic fatty acid-binding protein (H-FABP) in mediating whole body and muscle-specific long-chain fatty acid (LCFA) and glucose utilization was examined using exercise as a phenotyping tool. Catheters were chronically implanted in a carotid artery and jugular vein of wild-type (WT, n = 8), heterozygous (H-FABP+/-, n = 8), and null (H-FABP -/-, n = 7) chow-fed C57BL/6J mice, and mice were allowed to recover for 7 days. After a 5-h fast, conscious, unrestrained mice were studied during 30 min of treadmill exercise (0.6 mph). A bolus of [125I]-15-(p- iodophenyl)-3-R,S-methylpentadecanoic acid and 2-deoxy-[3H]glucose was administered to obtain rates of whole body metabolic clearance (MCR) and indexes of muscle LCFA (Rf) and glucose (Rg) utilization. Fasting, nonesterified fatty acids (mM) were elevated in H-FABP-/- mice (2.2 ± 0.9 vs. 1.3 ± 0.1 and 1.3 ± 0.2 for WT and H-FABP+/-). During exercise, blood glucose (mM) increased in WT (11.7 ± 0.8) and H-FABP+/- (12.6 ± 0.9) mice, whereas H-FABP-/- mice developed overt hypoglycemia (4.8 ± 0.8). Examination of tissue-specific and whole body glucose and LCFA utilization demonstrated a dependency on H-FABP with exercise in all tissues examined. Reductions in H-FABP led to decreasing exercise-stimulated Rf and increasing Rg with the most pronounced effects in heart and soleus muscle. Similar results were seen for MCR with decreasing LCFA and increasing glucose clearance with declining levels of H-FABP. These results show that, in vivo, H-FABP has reciprocal effects on glucose and LCFA utilization and whole body fuel homeostasis when metabolic demands are elevated by exercise.",
keywords = "15-(p-iodophenyl)-3-R,S-methylpentadecanoic acid, 2-deoxyglucose, Metabolism, Skeletal muscle, Substrate balance",
author = "Jane Shearer and Fueger, {Patrick T.} and Rottman, {Jeffrey N.} and Bracy, {Deanna P.} and Bert Binas and Wasserman, {David H.}",
year = "2005",
month = "2",
doi = "10.1152/ajpendo.00287.2004",
language = "English (US)",
volume = "288",
journal = "American Journal of Physiology - Renal Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "2 51-2",

}

TY - JOUR

T1 - Heart-type fatty acid-binding protein reciprocally regulates glucose and fatty acid utilization during exercise

AU - Shearer, Jane

AU - Fueger, Patrick T.

AU - Rottman, Jeffrey N.

AU - Bracy, Deanna P.

AU - Binas, Bert

AU - Wasserman, David H.

PY - 2005/2

Y1 - 2005/2

N2 - The role of heart-type cytosolic fatty acid-binding protein (H-FABP) in mediating whole body and muscle-specific long-chain fatty acid (LCFA) and glucose utilization was examined using exercise as a phenotyping tool. Catheters were chronically implanted in a carotid artery and jugular vein of wild-type (WT, n = 8), heterozygous (H-FABP+/-, n = 8), and null (H-FABP -/-, n = 7) chow-fed C57BL/6J mice, and mice were allowed to recover for 7 days. After a 5-h fast, conscious, unrestrained mice were studied during 30 min of treadmill exercise (0.6 mph). A bolus of [125I]-15-(p- iodophenyl)-3-R,S-methylpentadecanoic acid and 2-deoxy-[3H]glucose was administered to obtain rates of whole body metabolic clearance (MCR) and indexes of muscle LCFA (Rf) and glucose (Rg) utilization. Fasting, nonesterified fatty acids (mM) were elevated in H-FABP-/- mice (2.2 ± 0.9 vs. 1.3 ± 0.1 and 1.3 ± 0.2 for WT and H-FABP+/-). During exercise, blood glucose (mM) increased in WT (11.7 ± 0.8) and H-FABP+/- (12.6 ± 0.9) mice, whereas H-FABP-/- mice developed overt hypoglycemia (4.8 ± 0.8). Examination of tissue-specific and whole body glucose and LCFA utilization demonstrated a dependency on H-FABP with exercise in all tissues examined. Reductions in H-FABP led to decreasing exercise-stimulated Rf and increasing Rg with the most pronounced effects in heart and soleus muscle. Similar results were seen for MCR with decreasing LCFA and increasing glucose clearance with declining levels of H-FABP. These results show that, in vivo, H-FABP has reciprocal effects on glucose and LCFA utilization and whole body fuel homeostasis when metabolic demands are elevated by exercise.

AB - The role of heart-type cytosolic fatty acid-binding protein (H-FABP) in mediating whole body and muscle-specific long-chain fatty acid (LCFA) and glucose utilization was examined using exercise as a phenotyping tool. Catheters were chronically implanted in a carotid artery and jugular vein of wild-type (WT, n = 8), heterozygous (H-FABP+/-, n = 8), and null (H-FABP -/-, n = 7) chow-fed C57BL/6J mice, and mice were allowed to recover for 7 days. After a 5-h fast, conscious, unrestrained mice were studied during 30 min of treadmill exercise (0.6 mph). A bolus of [125I]-15-(p- iodophenyl)-3-R,S-methylpentadecanoic acid and 2-deoxy-[3H]glucose was administered to obtain rates of whole body metabolic clearance (MCR) and indexes of muscle LCFA (Rf) and glucose (Rg) utilization. Fasting, nonesterified fatty acids (mM) were elevated in H-FABP-/- mice (2.2 ± 0.9 vs. 1.3 ± 0.1 and 1.3 ± 0.2 for WT and H-FABP+/-). During exercise, blood glucose (mM) increased in WT (11.7 ± 0.8) and H-FABP+/- (12.6 ± 0.9) mice, whereas H-FABP-/- mice developed overt hypoglycemia (4.8 ± 0.8). Examination of tissue-specific and whole body glucose and LCFA utilization demonstrated a dependency on H-FABP with exercise in all tissues examined. Reductions in H-FABP led to decreasing exercise-stimulated Rf and increasing Rg with the most pronounced effects in heart and soleus muscle. Similar results were seen for MCR with decreasing LCFA and increasing glucose clearance with declining levels of H-FABP. These results show that, in vivo, H-FABP has reciprocal effects on glucose and LCFA utilization and whole body fuel homeostasis when metabolic demands are elevated by exercise.

KW - 15-(p-iodophenyl)-3-R,S-methylpentadecanoic acid

KW - 2-deoxyglucose

KW - Metabolism

KW - Skeletal muscle

KW - Substrate balance

UR - http://www.scopus.com/inward/record.url?scp=12144255122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12144255122&partnerID=8YFLogxK

U2 - 10.1152/ajpendo.00287.2004

DO - 10.1152/ajpendo.00287.2004

M3 - Article

VL - 288

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 1931-857X

IS - 2 51-2

ER -