Heat-induced modulation of lamin B content in two different cell lines

Wei Guo Zhu, Zachary V. Roberts, Joseph R. Dynlacht

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Previous work in our laboratory indicates that the nuclear matrix protein lamin B is a 'prompt' heat shock protein, which increases significantly when human U-1 melanoma and HeLa cells are exposed to 45.5°C for 5-40 min. Using Western blotting, we found that the lamin B content in U- 1 and HeLa cells increased to a greater extent during post-heat incubation at 37°C than during the heat dose itself. When HeLa cells were heated at 45.5°C for 30 min, and then incubated at 37°C for up to 7 h, lamin B content was increased significantly (1.69-fold maximum increase at 3 h) compared to unincubated heated cells. Also, thermotolerant HeLa cells showed a greater increase (up to 1.72-fold) in lamin B content during subsequent heating compared to nontolerant cells. The increase in lamin B content in thermotolerant cells, or when heated cells were incubated at 37°C, was also observed in U-1 cells. HeLa cells heated in the presence of glycerol (a heat protector) showed a 1.21-1.72-fold increase in lamin B content compared to cells heated for 10-30 min without glycerol. In contrast, lamin B content decreased 1.23-1.85-fold when cells were heated for 10-30 min in the presence of procaine (a heat sensitizer) compared to cells heated without procaine. These data suggest that lamin B may play an important role in the heat shock response, and that modulation of lamin B content by heat sensitizers or protectors may play a role in regulation of heat sensitivity.

Original languageEnglish (US)
Pages (from-to)620-628
Number of pages9
JournalJournal of Cellular Biochemistry
Volume75
Issue number4
DOIs
StatePublished - Dec 15 1999

Keywords

  • Heat shock
  • Lamin B
  • Nuclear matrix proteins
  • Thermotolerance

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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