Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1

Robert Hromas, Attilio Orazi, Richard S. Neiman, Richard Maki, Chuck Van Beveran, Jason Moore, Michael Klemsz

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

The ETS oncogene family member PU.1 is a transcriptional activator that is dysregulated by Friend erythroleukemia virus insertion. Northern analysis found that PU.1 is highly expressed in cells of myeloid and B-lymphoid origin, but not expressed at all in a number of nonhematopoietic tissues. Interferon-γ and retinoic acid downregulated PU.1 expression in marrow macrophages. In situ immunohistochemistry found that PU.1 is expressed only in early granulocytic and erythroid cells and megakaryocytes, but not in mature erythroid cells, mature granulocytes, endothelial cells, or osteocytes. Thus, its expression pattern makes PU.1 a candidate for a genetic determinant of lineage commitment and stage progression in blood cell development. It also lends insight into how PU.1 might play a role in Friend virus erythroleukemia.

Original languageEnglish (US)
Pages (from-to)2998-3004
Number of pages7
JournalBlood
Volume82
Issue number10
StatePublished - Nov 15 1993

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Friend murine leukemia virus
Leukemia, Erythroblastic, Acute
Erythroid Cells
Viruses
Oncogenes
Osteocytes
Megakaryocytes
Macrophages
Endothelial cells
Myeloid Cells
Tretinoin
Granulocytes
Interferons
Blood Cells
Blood
Down-Regulation
Endothelial Cells
Bone Marrow
Immunohistochemistry
Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Hromas, R., Orazi, A., Neiman, R. S., Maki, R., Van Beveran, C., Moore, J., & Klemsz, M. (1993). Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1. Blood, 82(10), 2998-3004.

Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1. / Hromas, Robert; Orazi, Attilio; Neiman, Richard S.; Maki, Richard; Van Beveran, Chuck; Moore, Jason; Klemsz, Michael.

In: Blood, Vol. 82, No. 10, 15.11.1993, p. 2998-3004.

Research output: Contribution to journalArticle

Hromas, R, Orazi, A, Neiman, RS, Maki, R, Van Beveran, C, Moore, J & Klemsz, M 1993, 'Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1', Blood, vol. 82, no. 10, pp. 2998-3004.
Hromas R, Orazi A, Neiman RS, Maki R, Van Beveran C, Moore J et al. Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1. Blood. 1993 Nov 15;82(10):2998-3004.
Hromas, Robert ; Orazi, Attilio ; Neiman, Richard S. ; Maki, Richard ; Van Beveran, Chuck ; Moore, Jason ; Klemsz, Michael. / Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1. In: Blood. 1993 ; Vol. 82, No. 10. pp. 2998-3004.
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