Hematopoietic potential of murine skeletal muscle-derived CD45-Sca-1+c-kit- cells

Jonathan C. Howell, Mervin C. Yoder, Edward F. Srour

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Objective: Somatic stem cells, which are poorly defined in postnatal mammalian tissues, are attractive candidates for examination of stem cell plasticity. Our goal was to determine the identity of neonatal muscle-derived cells that contain hematopoietic potential and to explore the status of CD45 expression on these cells. Materials and Methods: Skeletal muscle from thighs of 4- to 7-day-old mice was harvested, enzymatically digested, and flow cytometrically sorted to yield CD45-Sca-1+c-kit- cells. These cells were examined in hematopoietic colony-forming assays and competitive repopulation assays, and were expanded ex vivo. Additionally, CD45, c-kit, PU.1, and β globin major expression was tracked over time in cultured cells to assess the possibility of manipulating stem cell differentiation in vitro. Results: Freshly isolated CD45-Sca-1+c-kit- cells were devoid of hematopoietic lineage markers and contained no colony-forming activity but displayed superior long-term competitive repopulating ability when compared to freshly isolated muscle-derived CD45+Sca-1+c-kit+ cells. CD45-Sca-1+c-kit- cells expanded ex vivo in 5 ng/mL murine stem cell factor, mFlt-3L, and megakaryocyte growth and development factor (MGDF) for 9 days increased their in vivo hematopoietic repopulating potential 5.3-fold relative to fresh cells. Although fresh cells did not transcribe mRNA of several hematopoietic genes, a small fraction of cells cultured for 9 days acquired cell surface c-kit, and only these cells expressed c-kit and PU.1 mRNA and maintained competitive repopulating ability, suggesting at least myeloid and perhaps lymphoid developmental potential. Conclusion: Neonatal murine muscle-derived cells expressing the phenotype CD45-Sca-1+ c-kit- are putative adult somatic stem cells with in vitro and in vivo hematopoietic differentiation potential.

Original languageEnglish (US)
Pages (from-to)915-924
Number of pages10
JournalExperimental Hematology
Volume30
Issue number8
DOIs
StatePublished - Aug 14 2002

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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