Hematopoietic stem and progenitor cell mobilization in mice

Jonathan Hoggatt, Tiffany A. Tate, Louis M. Pelus

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

Hematopoietic stem cell transplantation (HSCT) can be performed with hematopoietic stem and progenitor cells (HSPC) acquired directly from bone marrow, from umbilical cord blood or placental tissue, or from the peripheral blood after treatment of the donor with agents that enhance egress of HSPC into the circulation, a process known as "mobilization." Mobilized peripheral blood stem cells (PBSC) have become the predominate hematopoietic graft for HSCT, particularly for autologous transplants. Despite the success of PBSC transplant, many patients and donors do not achieve optimal levels of mobilization. Thus, accurate animal models and basic laboratory investigations are needed to further investigate the mechanisms that lead to PBSC mobilization and define improved or new mobilizing agents and/or strategies to enhance PBSC mobilization and transplant. This chapter outlines assays and techniques for exploration of hematopoietic mobilization using mice as a model organism.

Original languageEnglish (US)
Title of host publicationHematopoietic Stem Cell Protocols
PublisherHumana Press Inc.
Pages43-64
Number of pages22
ISBN (Print)9781493911325
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1185
ISSN (Print)1064-3745

Keywords

  • Granulocyte-colony-stimulating factor (G-CSF)
  • Hematopoietic stem cell (HSC)
  • Mobilization
  • Mouse models

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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  • Cite this

    Hoggatt, J., Tate, T. A., & Pelus, L. M. (2014). Hematopoietic stem and progenitor cell mobilization in mice. In Hematopoietic Stem Cell Protocols (pp. 43-64). (Methods in Molecular Biology; Vol. 1185). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-1133-2_4