Hemodynamics in transgenic mice with overexpression of atrial natriuretic factor

R. Wayne Barbee, Bret D. Perry, Richard N. Ré, Joseph P. Murgo, Loren Field

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The circulatory effects associated with lifelong plasma atrial natriuretic factor (ANF) elevation were examined by generating transgenic mice, which constitutively express a fusion gene consisting of the transthyretin promoter and the ANF structural gene. These mice have chronically elevated ANF levels as compared with their nontransgenic siblings. Transgenic animals exhibited immunoreactive ANF levels that were nearly fivefold higher than those measured in nontransgenic littermates. Systemic and regional hemodynamics and blood volumes were explored by using modifications of the reference microsphere and dilution techniques. Mean arterial pressure was reduced by 24 mm Hg, associated with a 27% reduction in total heart weight. This chronic reduction in blood pressure was due to a 21% reduction in total peripheral resistance, whereas cardiac output, stroke volume, and heart rate were not significantly altered, despite a 15% elevation in plasma volume. Transgenic mice displayed reductions of 35%, 33%, 32%, and 19% in muscle, skin, brain, and renal vascular resistance, respectively, whereas coronary and splanchnic resistances were not significantly altered. The findings complement earlier data from chronically infused normotensive mammals and suggest that these mice are an excellent model for investigating the effects of lifelong ANF elevation.

Original languageEnglish
Pages (from-to)747-751
Number of pages5
JournalCirculation Research
Volume74
Issue number4
StatePublished - Apr 1994

Fingerprint

Atrial Natriuretic Factor
Transgenic Mice
Hemodynamics
Vascular Resistance
Indicator Dilution Techniques
Genetically Modified Animals
Prealbumin
Viscera
Plasma Volume
Gene Fusion
Blood Volume
Microspheres
Cardiac Output
Stroke Volume
Mammals
Arterial Pressure
Heart Rate
Blood Pressure
Kidney
Weights and Measures

Keywords

  • atrial natriuretic factor
  • autoregulation
  • blood pressure
  • blood volume
  • cardiac output
  • vascular resistance

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Barbee, R. W., Perry, B. D., Ré, R. N., Murgo, J. P., & Field, L. (1994). Hemodynamics in transgenic mice with overexpression of atrial natriuretic factor. Circulation Research, 74(4), 747-751.

Hemodynamics in transgenic mice with overexpression of atrial natriuretic factor. / Barbee, R. Wayne; Perry, Bret D.; Ré, Richard N.; Murgo, Joseph P.; Field, Loren.

In: Circulation Research, Vol. 74, No. 4, 04.1994, p. 747-751.

Research output: Contribution to journalArticle

Barbee, RW, Perry, BD, Ré, RN, Murgo, JP & Field, L 1994, 'Hemodynamics in transgenic mice with overexpression of atrial natriuretic factor', Circulation Research, vol. 74, no. 4, pp. 747-751.
Barbee, R. Wayne ; Perry, Bret D. ; Ré, Richard N. ; Murgo, Joseph P. ; Field, Loren. / Hemodynamics in transgenic mice with overexpression of atrial natriuretic factor. In: Circulation Research. 1994 ; Vol. 74, No. 4. pp. 747-751.
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