Heparin-binding proteins are involved in thrombin time variability in normal and patient plasmas

H. Xiao, Steven Miller, W. P. Faulk

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The ability of plasma proteins to neutralize the anticoagulant activity of heparin was studied using a thrombin time assay with 20 normal adult and 207 patient plasma samples. The thrombin times of normal adult plasmas spiked with the same amount of heparin were found to vary significantly, and this variability was attributed to differences in heparin-binding proteins among individuals. This possibility was investigated by determining thrombin times for a variety of plasma samples following proteolytic digestion. A study of patient pooled plasma showed that incubation with a protease increased the thrombin time from 23 s to > 300 s, suggesting that the anticoagulant activity of heparin could be neutralized by plasma proteins and released with proteolytic digestion. Incubation of the digested plasma with heparinase I returned the thrombin time almost to control values. In addition, patients who received prior heparin injections had different responses to similar dosages of heparin, as indicated by differences in thrombin times. Thus, the anticoagulant activity of heparin may be affected by the balance between free heparin and protein-bound heparin in plasma. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)455-460
Number of pages6
JournalBlood Coagulation and Fibrinolysis
Volume11
Issue number5
DOIs
StatePublished - 2000

Fingerprint

Thrombin Time
Heparin
Anticoagulants
Blood Proteins
Digestion
Heparin Lyase
human cationic antimicrobial protein CAP 37
Peptide Hydrolases
Injections

Keywords

  • Heparin
  • Heparin-binding proteins
  • Plasma
  • Thrombin time

ASJC Scopus subject areas

  • Hematology

Cite this

Heparin-binding proteins are involved in thrombin time variability in normal and patient plasmas. / Xiao, H.; Miller, Steven; Faulk, W. P.

In: Blood Coagulation and Fibrinolysis, Vol. 11, No. 5, 2000, p. 455-460.

Research output: Contribution to journalArticle

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AB - The ability of plasma proteins to neutralize the anticoagulant activity of heparin was studied using a thrombin time assay with 20 normal adult and 207 patient plasma samples. The thrombin times of normal adult plasmas spiked with the same amount of heparin were found to vary significantly, and this variability was attributed to differences in heparin-binding proteins among individuals. This possibility was investigated by determining thrombin times for a variety of plasma samples following proteolytic digestion. A study of patient pooled plasma showed that incubation with a protease increased the thrombin time from 23 s to > 300 s, suggesting that the anticoagulant activity of heparin could be neutralized by plasma proteins and released with proteolytic digestion. Incubation of the digested plasma with heparinase I returned the thrombin time almost to control values. In addition, patients who received prior heparin injections had different responses to similar dosages of heparin, as indicated by differences in thrombin times. Thus, the anticoagulant activity of heparin may be affected by the balance between free heparin and protein-bound heparin in plasma. (C) 2000 Lippincott Williams and Wilkins.

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