Hepatic branched-chain α-keto acid dehydrogenase complex in female rats

Activation by exercise and starvation

Rumi Kobayashi, Yoshiharu Shimomura, Taro Murakami, Naoya Nakai, Megumi Otsuka, Nobuhiko Arakawa, Kazuyuki Shimizu, Robert Harris

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The effects of acute exercise and starvation on hepatic branched-chain α-keto acid dehydrogenase (BCKDH) complex activity were examined in female rats fed high (30%)- or low (8%)-protein diets. The total activity of the complex was significantly higher in the high protein-fed rats than in the low protein-fed rats but was not affected by acute exercise and starvation in either diet group. The proportion of the active form of BCKDH complex was less than 10% in both diet groups. Acute exercise and starvation markedly increased the active form of the complex in both diet groups. The activity of BCKDH kinase, which is responsible for inactivation of the BCKDH complex by phosphorylation, tended to be decreased by acute exercise and starvation in both diet groups. These results suggest that the activity of the BCKDH kinase is an important factor determining the proportion of the active form of BCKDH complex in exercise and starvation, and that the female rat is a useful model for studying the regulation of hepatic BCKDH complex activity.

Original languageEnglish
Pages (from-to)303-309
Number of pages7
JournalJournal of Nutritional Science and Vitaminology
Volume45
Issue number3
StatePublished - 1999

Fingerprint

3-methyl-2-oxobutanoate dehydrogenase (lipoamide)
3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Starvation
starvation
exercise
liver
Liver
rats
Exercise
Diet
diet
phosphotransferases (kinases)
Phosphotransferases
Protein-Restricted Diet
dietary protein
phosphorylation
inactivation
proteins
Phosphorylation

Keywords

  • BCKDH kinase
  • Branched-chain α-keto acid dehydrogenase (BCKDH) complex
  • Exercise
  • Female rat liver
  • Starvation

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Hepatic branched-chain α-keto acid dehydrogenase complex in female rats : Activation by exercise and starvation. / Kobayashi, Rumi; Shimomura, Yoshiharu; Murakami, Taro; Nakai, Naoya; Otsuka, Megumi; Arakawa, Nobuhiko; Shimizu, Kazuyuki; Harris, Robert.

In: Journal of Nutritional Science and Vitaminology, Vol. 45, No. 3, 1999, p. 303-309.

Research output: Contribution to journalArticle

Kobayashi, R, Shimomura, Y, Murakami, T, Nakai, N, Otsuka, M, Arakawa, N, Shimizu, K & Harris, R 1999, 'Hepatic branched-chain α-keto acid dehydrogenase complex in female rats: Activation by exercise and starvation', Journal of Nutritional Science and Vitaminology, vol. 45, no. 3, pp. 303-309.
Kobayashi, Rumi ; Shimomura, Yoshiharu ; Murakami, Taro ; Nakai, Naoya ; Otsuka, Megumi ; Arakawa, Nobuhiko ; Shimizu, Kazuyuki ; Harris, Robert. / Hepatic branched-chain α-keto acid dehydrogenase complex in female rats : Activation by exercise and starvation. In: Journal of Nutritional Science and Vitaminology. 1999 ; Vol. 45, No. 3. pp. 303-309.
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