Hepatic gene transfer in animals using retroviruses containing the promoter from the gene for phosphoenolpyruvate carboxykinase

Maria Hatzoglou, Wouter Lamers, Fatima Bosch, Anthony Wynshaw-Boris, D. Clapp, Richard W. Hanson

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Two methods are described for directing the expression of genes to the livers of animals using retroviral vectors containing the predominantly liver-specific promoter from the gene for phosphoenolpyruvate carboxykinase (PEPCK)-linked to the structural gene for either amino 3′-glycosyl phosphotransferase (neo) or bovine growth hormone (bGH). Replication-incompetent retrovirus was used to infect the livers of fetal rats by intraperitoneal injection of animals in utero or to infect adult rats by direct injection into the portal vein after partial hepatectomy. The proviruses were integrated into the hepatic DNA, and the chimeric genes were expressed from the PEPCK promoter for as long as 8 months after infection. The expression of the PEPCK-bGH gene was regulated by diet and hormones in a manner similar to the regulation of the endogenous PEPCK gene in the liver. The potential of this method for targeting genes to the liver is discussed.

Original languageEnglish (US)
Pages (from-to)17285-17293
Number of pages9
JournalJournal of Biological Chemistry
Volume265
Issue number28
StatePublished - Oct 5 1990
Externally publishedYes

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Gene transfer
Phosphoenolpyruvate
Retroviridae
Animals
Genes
Liver
Rats
Proviruses
Gene Targeting
Hepatectomy
Portal Vein
Intraperitoneal Injections
Direct injection
Nutrition
Phosphotransferases
Hormones
Diet
Gene Expression
Injections
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hepatic gene transfer in animals using retroviruses containing the promoter from the gene for phosphoenolpyruvate carboxykinase. / Hatzoglou, Maria; Lamers, Wouter; Bosch, Fatima; Wynshaw-Boris, Anthony; Clapp, D.; Hanson, Richard W.

In: Journal of Biological Chemistry, Vol. 265, No. 28, 05.10.1990, p. 17285-17293.

Research output: Contribution to journalArticle

Hatzoglou, Maria ; Lamers, Wouter ; Bosch, Fatima ; Wynshaw-Boris, Anthony ; Clapp, D. ; Hanson, Richard W. / Hepatic gene transfer in animals using retroviruses containing the promoter from the gene for phosphoenolpyruvate carboxykinase. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 28. pp. 17285-17293.
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