Hepatic protein pattern alterations following perfluorodecanoic acid exposure in rats

Frank Witzmann, D. N. Parker

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effect after 8 days of 20 and 50 mg/kg in vivo exposure to perfluoro-n-decanoic acid (PFDA) on the protein pattern of rat liver whole homogenates was studied using high-resolution, large-scale two-dimensional polyacrylamide gel electrophoresis. PFDA exposure altered 13 proteins reproducibly in each of 5 samples tested. While most of the altered proteins remained unidentified, several known mitochondrial proteins were induced by PFDA as were others in a dose-related manner. Conversely, PFDA caused the reduction of albumin as well as several unknown proteins, also in a dose-related manner. Cytoskeletal proteins were unaffected by PFDA. The present results suggest that PFDA's toxic mechanism may involve more than membrane integration and lipid accumulation. Changes in the two-dimensional protein pattern indicate that selective protein synthetic or catabolic processes may undergo qualitative alterations after PFDA treatment as well.

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalToxicology Letters
Volume57
Issue number1
DOIs
StatePublished - 1991

Fingerprint

Rats
Liver
Proteins
Cytoskeletal Proteins
Poisons
Mitochondrial Proteins
Electrophoresis, Gel, Two-Dimensional
Membrane Lipids
Electrophoresis
perfluorodecanoic acid
Albumins
Membranes
Lipids

Keywords

  • Perfluorochemical toxicity
  • Two-dimensional polyacrylamide gel electrophoresis
  • Whole homogenates

ASJC Scopus subject areas

  • Toxicology

Cite this

Hepatic protein pattern alterations following perfluorodecanoic acid exposure in rats. / Witzmann, Frank; Parker, D. N.

In: Toxicology Letters, Vol. 57, No. 1, 1991, p. 29-36.

Research output: Contribution to journalArticle

@article{7d5af68075c14acb99d8b230fdd4640f,
title = "Hepatic protein pattern alterations following perfluorodecanoic acid exposure in rats",
abstract = "The effect after 8 days of 20 and 50 mg/kg in vivo exposure to perfluoro-n-decanoic acid (PFDA) on the protein pattern of rat liver whole homogenates was studied using high-resolution, large-scale two-dimensional polyacrylamide gel electrophoresis. PFDA exposure altered 13 proteins reproducibly in each of 5 samples tested. While most of the altered proteins remained unidentified, several known mitochondrial proteins were induced by PFDA as were others in a dose-related manner. Conversely, PFDA caused the reduction of albumin as well as several unknown proteins, also in a dose-related manner. Cytoskeletal proteins were unaffected by PFDA. The present results suggest that PFDA's toxic mechanism may involve more than membrane integration and lipid accumulation. Changes in the two-dimensional protein pattern indicate that selective protein synthetic or catabolic processes may undergo qualitative alterations after PFDA treatment as well.",
keywords = "Perfluorochemical toxicity, Two-dimensional polyacrylamide gel electrophoresis, Whole homogenates",
author = "Frank Witzmann and Parker, {D. N.}",
year = "1991",
doi = "10.1016/0378-4274(91)90116-N",
language = "English",
volume = "57",
pages = "29--36",
journal = "Toxicology Letters",
issn = "0378-4274",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Hepatic protein pattern alterations following perfluorodecanoic acid exposure in rats

AU - Witzmann, Frank

AU - Parker, D. N.

PY - 1991

Y1 - 1991

N2 - The effect after 8 days of 20 and 50 mg/kg in vivo exposure to perfluoro-n-decanoic acid (PFDA) on the protein pattern of rat liver whole homogenates was studied using high-resolution, large-scale two-dimensional polyacrylamide gel electrophoresis. PFDA exposure altered 13 proteins reproducibly in each of 5 samples tested. While most of the altered proteins remained unidentified, several known mitochondrial proteins were induced by PFDA as were others in a dose-related manner. Conversely, PFDA caused the reduction of albumin as well as several unknown proteins, also in a dose-related manner. Cytoskeletal proteins were unaffected by PFDA. The present results suggest that PFDA's toxic mechanism may involve more than membrane integration and lipid accumulation. Changes in the two-dimensional protein pattern indicate that selective protein synthetic or catabolic processes may undergo qualitative alterations after PFDA treatment as well.

AB - The effect after 8 days of 20 and 50 mg/kg in vivo exposure to perfluoro-n-decanoic acid (PFDA) on the protein pattern of rat liver whole homogenates was studied using high-resolution, large-scale two-dimensional polyacrylamide gel electrophoresis. PFDA exposure altered 13 proteins reproducibly in each of 5 samples tested. While most of the altered proteins remained unidentified, several known mitochondrial proteins were induced by PFDA as were others in a dose-related manner. Conversely, PFDA caused the reduction of albumin as well as several unknown proteins, also in a dose-related manner. Cytoskeletal proteins were unaffected by PFDA. The present results suggest that PFDA's toxic mechanism may involve more than membrane integration and lipid accumulation. Changes in the two-dimensional protein pattern indicate that selective protein synthetic or catabolic processes may undergo qualitative alterations after PFDA treatment as well.

KW - Perfluorochemical toxicity

KW - Two-dimensional polyacrylamide gel electrophoresis

KW - Whole homogenates

UR - http://www.scopus.com/inward/record.url?scp=0025735117&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025735117&partnerID=8YFLogxK

U2 - 10.1016/0378-4274(91)90116-N

DO - 10.1016/0378-4274(91)90116-N

M3 - Article

VL - 57

SP - 29

EP - 36

JO - Toxicology Letters

JF - Toxicology Letters

SN - 0378-4274

IS - 1

ER -