Hepatocellular proliferation and changes in microarchitecture of right lobe allografts in adult transplant recipients

Ruoqing Huang, Thomas D. Schiano, May Jennifer Amolat, Charles M. Miller, Swan N. Thung, Romil Saxena

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Abstract

Imaging studies show complete restoration of liver volume in adult recipients of right lobe allografts within 2 - 3 weeks of living donor transplantation (LDLT). However, it is not known if this growth is associated with restoration of hepatic microarchitecture. We compared 21 biopsies without significant pathology from LDLT recipients with 23 biopsies from adult recipients of cadaveric donor liver transplantation (CDLT) performed within 3 months of transplantation. The difference in the number of portal tracts per cm was statistically significant (P < .0001) between CDLT (9.08 ± 1.74) and LDLT recipients within 3 months (6.26 ± 1.62), as well as after 3 months following transplantation (6.56 ± 1.44). The coefficient of correlation between length of biopsy specimens and the number of portal tracts in these 3 groups was .94,.93, and .85, respectively. Proliferative activity demonstrated by immunohistochemical staining for MIB-1 was seen predominantly in hepatocytes in both groups; bile ducts only occasionally stained positive. The difference between labeling indices of hepatocytes was statistically significant (P = .00056) between CDLT and LDLT recipients within 3 months of transplantation (.82 ± .63 and 4.53 ± 3.72), and between LDLT recipients within 3 weeks and after 3 weeks of transplantation (5.97 ± 3.78 and 1.80 ± 1.37, P = .0074). In conclusion, restoration of liver volume following LDLT occurs by proliferation of hepatocytes in the immediate posttransplant period. There is a decrease in number of portal tracts in these volume-restored allografts. Volume restoration is therefore, not accompanied by restoration of hepatic microarchitecture.

Original languageEnglish
Pages (from-to)1461-1467
Number of pages7
JournalLiver Transplantation
Volume10
Issue number12
DOIs
StatePublished - Dec 2004

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Allografts
Transplantation
Living Donors
Liver Transplantation
Hepatocytes
Liver
Tissue Donors
Biopsy
Transplant Recipients
Bile Ducts
Pathology
Staining and Labeling
Growth

ASJC Scopus subject areas

  • Surgery
  • Transplantation

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Hepatocellular proliferation and changes in microarchitecture of right lobe allografts in adult transplant recipients. / Huang, Ruoqing; Schiano, Thomas D.; Amolat, May Jennifer; Miller, Charles M.; Thung, Swan N.; Saxena, Romil.

In: Liver Transplantation, Vol. 10, No. 12, 12.2004, p. 1461-1467.

Research output: Contribution to journalArticle

Huang, Ruoqing ; Schiano, Thomas D. ; Amolat, May Jennifer ; Miller, Charles M. ; Thung, Swan N. ; Saxena, Romil. / Hepatocellular proliferation and changes in microarchitecture of right lobe allografts in adult transplant recipients. In: Liver Transplantation. 2004 ; Vol. 10, No. 12. pp. 1461-1467.
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