HER-2/neu expression in germ cell tumours

S. Soule, K. Miller, L. Baldridge, K. Kirkpatrick, L. Cheng, J. L. Gilbert, L. R. Smith, V. C. Thurston, G. H. Vance, L. Einhorn

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Abstract

Aims: To determine the rate of HER-2/neu positivity of germ cell tumours by immunohistochemistry (IHC) and by fluorescence in situ hybridisation (FISH). Patients/Methods: Ninety six archival, paraffin wax embedded pathology specimens were chosen from four groups of germ cell tumours. IHC for HER-2/neu was performed with the HercepTest kit; FISH analysis was performed with the INFORM assay and confirmed with a centromere 17 probe. Results: Twenty two of 96 specimens overexpressed the HER-2/neu protein when measured by IHC. Only three specimens showed HER-2/neu gene amplification by FISH. There was no correlation between the results obtained by IHC and FISH. Conclusions: The lack of concordance between IHC and FISH makes it unlikely that overexpression of the HER-2/neu protein in germ cell tumours is of prognostic or therapeutic relevance. Because of the low rate of HER-2/neu gene amplification in germ cell tumours, a clinical trial of trastuzumab treatment in patients with germ cell tumours is not warranted.

Original languageEnglish (US)
Pages (from-to)656-658
Number of pages3
JournalJournal of clinical pathology
Volume55
Issue number9
DOIs
StatePublished - Sep 1 2002

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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Soule, S., Miller, K., Baldridge, L., Kirkpatrick, K., Cheng, L., Gilbert, J. L., Smith, L. R., Thurston, V. C., Vance, G. H., & Einhorn, L. (2002). HER-2/neu expression in germ cell tumours. Journal of clinical pathology, 55(9), 656-658. https://doi.org/10.1136/jcp.55.9.656