Heterogeneity among resident murine peritoneal macrophages: Separation and functional characterization of monocytoid cells producing granulocyte-macrophage colony-stimulating factor (GM-CSF) and responding to regulation by lactoferrin

Louis Pelus, Hal Broxmeyer, M. DeSousa, M. A S Moore

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Abstract

Analysis of GM-CSF production by unstimulated, resident murine peritoneal cells after velocity sedimentation separation indicated the presence of 2 GM-CSF-producing monocytoid cell populations. The 1st population of cells sedimented over a range of 5.8 to 8.0 mm/hr and consisted primarily of large and medium-sized adherent, phagocytic, nonspecific esterase-positive, Fc receptor-bearing monocytes and macrophages. The 2nd GM-CSF-producing cell population had a mean sedimentation velocity of 4.0 mm/hr and consisted of Fc receptor-positive, weakly phagocytic, esterase-positive, Thy-1.2-negative, GM-CSF-producing cells, which contained little detectable acid hydrolase or lysozyme activity. Morphologically, these cells appeared to consist primarily of small monocytes and less differentiated monocytoid-like cells. In the presence of iron-saturated lactoferrin, constitutive GM-CSF production was inhibited in those monocytoid cells sedimenting at 5.8 to 8.0 mm/hr but not in those sedimenting at 4.0 mm/hr. The ability of lactoferrin to inhibit GM-CSF production correlated directly with the ability of those cells to bind lactoferrin. The addition of bacterial lipopolysaccharide reversed the inhibitory effect of lactoferrin on the 5.8 to 8.0 mm/hr monocyte-macrophage population and returned GM-CSF production to control levels. No effect of LPS was observed on the 4.0 mm/hr cell population; however, enhancement of GM-CSF production from cells sedimenting at 1.0 to 3.0 mm/hr was noted. Lactoferrin was without effect on the production of a monocyte-macrophage-derived stimulating factor necessary for the generation of CFU-GM in suspension culture by either cell population. These results indicate that the regulation of monocyte-macrophage-derived hematopoietic relevant growth factors is complex and depends upon specific regulatory signals as well as specific subpopulations of monocytoid cells.

Original languageEnglish (US)
Pages (from-to)1016-1021
Number of pages6
JournalJournal of Immunology
Volume126
Issue number3
StatePublished - 1981
Externally publishedYes

Fingerprint

Lactoferrin
Peritoneal Macrophages
Granulocyte-Macrophage Colony-Stimulating Factor
Macrophages
Population
Monocytes
Fc Receptors
Carboxylesterase
Granulocyte-Macrophage Progenitor Cells
Hydrolases
Esterases
Muramidase
Lipopolysaccharides
Intercellular Signaling Peptides and Proteins
Suspensions
Iron
Cell Culture Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

@article{6945736d7f944b4b93908a83208f7c60,
title = "Heterogeneity among resident murine peritoneal macrophages: Separation and functional characterization of monocytoid cells producing granulocyte-macrophage colony-stimulating factor (GM-CSF) and responding to regulation by lactoferrin",
abstract = "Analysis of GM-CSF production by unstimulated, resident murine peritoneal cells after velocity sedimentation separation indicated the presence of 2 GM-CSF-producing monocytoid cell populations. The 1st population of cells sedimented over a range of 5.8 to 8.0 mm/hr and consisted primarily of large and medium-sized adherent, phagocytic, nonspecific esterase-positive, Fc receptor-bearing monocytes and macrophages. The 2nd GM-CSF-producing cell population had a mean sedimentation velocity of 4.0 mm/hr and consisted of Fc receptor-positive, weakly phagocytic, esterase-positive, Thy-1.2-negative, GM-CSF-producing cells, which contained little detectable acid hydrolase or lysozyme activity. Morphologically, these cells appeared to consist primarily of small monocytes and less differentiated monocytoid-like cells. In the presence of iron-saturated lactoferrin, constitutive GM-CSF production was inhibited in those monocytoid cells sedimenting at 5.8 to 8.0 mm/hr but not in those sedimenting at 4.0 mm/hr. The ability of lactoferrin to inhibit GM-CSF production correlated directly with the ability of those cells to bind lactoferrin. The addition of bacterial lipopolysaccharide reversed the inhibitory effect of lactoferrin on the 5.8 to 8.0 mm/hr monocyte-macrophage population and returned GM-CSF production to control levels. No effect of LPS was observed on the 4.0 mm/hr cell population; however, enhancement of GM-CSF production from cells sedimenting at 1.0 to 3.0 mm/hr was noted. Lactoferrin was without effect on the production of a monocyte-macrophage-derived stimulating factor necessary for the generation of CFU-GM in suspension culture by either cell population. These results indicate that the regulation of monocyte-macrophage-derived hematopoietic relevant growth factors is complex and depends upon specific regulatory signals as well as specific subpopulations of monocytoid cells.",
author = "Louis Pelus and Hal Broxmeyer and M. DeSousa and Moore, {M. A S}",
year = "1981",
language = "English (US)",
volume = "126",
pages = "1016--1021",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

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T1 - Heterogeneity among resident murine peritoneal macrophages

T2 - Separation and functional characterization of monocytoid cells producing granulocyte-macrophage colony-stimulating factor (GM-CSF) and responding to regulation by lactoferrin

AU - Pelus, Louis

AU - Broxmeyer, Hal

AU - DeSousa, M.

AU - Moore, M. A S

PY - 1981

Y1 - 1981

N2 - Analysis of GM-CSF production by unstimulated, resident murine peritoneal cells after velocity sedimentation separation indicated the presence of 2 GM-CSF-producing monocytoid cell populations. The 1st population of cells sedimented over a range of 5.8 to 8.0 mm/hr and consisted primarily of large and medium-sized adherent, phagocytic, nonspecific esterase-positive, Fc receptor-bearing monocytes and macrophages. The 2nd GM-CSF-producing cell population had a mean sedimentation velocity of 4.0 mm/hr and consisted of Fc receptor-positive, weakly phagocytic, esterase-positive, Thy-1.2-negative, GM-CSF-producing cells, which contained little detectable acid hydrolase or lysozyme activity. Morphologically, these cells appeared to consist primarily of small monocytes and less differentiated monocytoid-like cells. In the presence of iron-saturated lactoferrin, constitutive GM-CSF production was inhibited in those monocytoid cells sedimenting at 5.8 to 8.0 mm/hr but not in those sedimenting at 4.0 mm/hr. The ability of lactoferrin to inhibit GM-CSF production correlated directly with the ability of those cells to bind lactoferrin. The addition of bacterial lipopolysaccharide reversed the inhibitory effect of lactoferrin on the 5.8 to 8.0 mm/hr monocyte-macrophage population and returned GM-CSF production to control levels. No effect of LPS was observed on the 4.0 mm/hr cell population; however, enhancement of GM-CSF production from cells sedimenting at 1.0 to 3.0 mm/hr was noted. Lactoferrin was without effect on the production of a monocyte-macrophage-derived stimulating factor necessary for the generation of CFU-GM in suspension culture by either cell population. These results indicate that the regulation of monocyte-macrophage-derived hematopoietic relevant growth factors is complex and depends upon specific regulatory signals as well as specific subpopulations of monocytoid cells.

AB - Analysis of GM-CSF production by unstimulated, resident murine peritoneal cells after velocity sedimentation separation indicated the presence of 2 GM-CSF-producing monocytoid cell populations. The 1st population of cells sedimented over a range of 5.8 to 8.0 mm/hr and consisted primarily of large and medium-sized adherent, phagocytic, nonspecific esterase-positive, Fc receptor-bearing monocytes and macrophages. The 2nd GM-CSF-producing cell population had a mean sedimentation velocity of 4.0 mm/hr and consisted of Fc receptor-positive, weakly phagocytic, esterase-positive, Thy-1.2-negative, GM-CSF-producing cells, which contained little detectable acid hydrolase or lysozyme activity. Morphologically, these cells appeared to consist primarily of small monocytes and less differentiated monocytoid-like cells. In the presence of iron-saturated lactoferrin, constitutive GM-CSF production was inhibited in those monocytoid cells sedimenting at 5.8 to 8.0 mm/hr but not in those sedimenting at 4.0 mm/hr. The ability of lactoferrin to inhibit GM-CSF production correlated directly with the ability of those cells to bind lactoferrin. The addition of bacterial lipopolysaccharide reversed the inhibitory effect of lactoferrin on the 5.8 to 8.0 mm/hr monocyte-macrophage population and returned GM-CSF production to control levels. No effect of LPS was observed on the 4.0 mm/hr cell population; however, enhancement of GM-CSF production from cells sedimenting at 1.0 to 3.0 mm/hr was noted. Lactoferrin was without effect on the production of a monocyte-macrophage-derived stimulating factor necessary for the generation of CFU-GM in suspension culture by either cell population. These results indicate that the regulation of monocyte-macrophage-derived hematopoietic relevant growth factors is complex and depends upon specific regulatory signals as well as specific subpopulations of monocytoid cells.

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