Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse: Roles of osteopontin

Jinyi Shao, M. Kay Washington, Romil Saxena, Hongmiao Sheng

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The persistent activation of the phosphatidylinositol 3-kinase (PI3K)/ Akt pathway is oncogenic and involved in colorectal neoplasia. Mutations of both regulatory subunit and catalytic subunit of PI3K have been demonstrated in colon cancers. In the present study, we show that heterozygous disruption of the phosphatase and tensin homolog (PTEN) tumor suppressor gene promoted tumor progression in APCmin/+ mice. Number and size of intestinal tumors were significantly increased in mice bearing both adenomatous polyposis coli (APC) and PTEN mutations. While APCmin/+PTEN+/+ mice developed adenomas, invasive carcinomas developed in APCmin/+ PTEN+/- mice. Large tumors often resulted in intestinal intussusception and early death of APCmin/+PTEN+/- mice. Targeted array revealed that osteopontin (OPN) was the leading gene whose expression was strongly induced by deficiency of PTEN. In colon cancer cells, gain-of-function mutation of PI3K robustly increased levels of OPN and treatment with OPN reduced growth factor deprivation-induced programmed cell death. Moreover, OPN expression was strongly increased in Ras-induced transformation of intestinal epithelial cells in a PI3K-dependent manner. Inhibition of OPN expression by specific small interfering RNA reduced uncontrolled growth and invasiveness of Ras-transformed intestinal epithelial cells. Thus, our results suggest that the PI3K pathway promotes the transformation of intestinal adenoma to adenocarcinoma. OPN, a downstream effector of PI3K, protects transformed intestinal epithelial cells from programmed cell death and stimulates their anchorage-independent growth.

Original languageEnglish
Pages (from-to)2476-2483
Number of pages8
JournalCarcinogenesis
Volume28
Issue number12
DOIs
StatePublished - Dec 2007

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Phosphatidylinositol 3-Kinase
Osteopontin
Phosphoric Monoester Hydrolases
Neoplasms
Epithelial Cells
Adenoma
Colonic Neoplasms
Mutation
Cell Death
Adenomatous Polyposis Coli
Intussusception
Growth
Tumor Suppressor Genes
Small Interfering RNA
Tensins
Catalytic Domain
Intercellular Signaling Peptides and Proteins
Adenocarcinoma
Carcinoma
Gene Expression

ASJC Scopus subject areas

  • Cancer Research

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Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse : Roles of osteopontin. / Shao, Jinyi; Washington, M. Kay; Saxena, Romil; Sheng, Hongmiao.

In: Carcinogenesis, Vol. 28, No. 12, 12.2007, p. 2476-2483.

Research output: Contribution to journalArticle

Shao, Jinyi ; Washington, M. Kay ; Saxena, Romil ; Sheng, Hongmiao. / Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse : Roles of osteopontin. In: Carcinogenesis. 2007 ; Vol. 28, No. 12. pp. 2476-2483.
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abstract = "The persistent activation of the phosphatidylinositol 3-kinase (PI3K)/ Akt pathway is oncogenic and involved in colorectal neoplasia. Mutations of both regulatory subunit and catalytic subunit of PI3K have been demonstrated in colon cancers. In the present study, we show that heterozygous disruption of the phosphatase and tensin homolog (PTEN) tumor suppressor gene promoted tumor progression in APCmin/+ mice. Number and size of intestinal tumors were significantly increased in mice bearing both adenomatous polyposis coli (APC) and PTEN mutations. While APCmin/+PTEN+/+ mice developed adenomas, invasive carcinomas developed in APCmin/+ PTEN+/- mice. Large tumors often resulted in intestinal intussusception and early death of APCmin/+PTEN+/- mice. Targeted array revealed that osteopontin (OPN) was the leading gene whose expression was strongly induced by deficiency of PTEN. In colon cancer cells, gain-of-function mutation of PI3K robustly increased levels of OPN and treatment with OPN reduced growth factor deprivation-induced programmed cell death. Moreover, OPN expression was strongly increased in Ras-induced transformation of intestinal epithelial cells in a PI3K-dependent manner. Inhibition of OPN expression by specific small interfering RNA reduced uncontrolled growth and invasiveness of Ras-transformed intestinal epithelial cells. Thus, our results suggest that the PI3K pathway promotes the transformation of intestinal adenoma to adenocarcinoma. OPN, a downstream effector of PI3K, protects transformed intestinal epithelial cells from programmed cell death and stimulates their anchorage-independent growth.",
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