High dietary carbohydrates decrease gallbladder volume and enhance cholesterol crystal formation

Abhishek Mathur, Marine Megan, Hayder H. Al-Azzawi, Debao Lu, Deborah A. Swartz-Basile, Attila Nakeeb, Henry A. Pitt

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Animal and human data suggest that a diet high in refined carbohydrates leads to gallstone formation. However, no data are available on the role of dietary carbohydrates on gallbladder volume or on cholesterol crystal formation. Therefore, we tested the hypothesis that a high carbohydrate diet would alter gallbladder volume and enhance cholesterol crystal formation. Methods: At 8 weeks of age, 60 lean and 36 obese leptin-deficient female mice were fed a 45% carbohydrate diet while an equal number of lean and obese mice were fed a 75% carbohydrate diet for 4 weeks. All animals then underwent cholecystectomy, and gallbladder bile volume was recorded. Bile was pooled, filtered, and maintained in a water bath at 37°C for 14 days. Birefringent cholesterol crystals in bile were counted daily; crystal observation time and crystal mass were determined. Results: The crystal observation time was significantly shortened in both lean and obese mice on the 75% diet compared with their counterparts on the 45% diet. The crystal mass was significantly increased in the lean mice on the 75% diet compared with the 45% diet. Gallbladder volumes were significantly reduced in both lean and obese mice on the 75% diet compared with their counterparts on the 45% diet. Conclusions: These data suggest that a high carbohydrate diet decreases gallbladder volume, shortens cholesterol crystal observation time, and increases crystal mass. We conclude that dietary carbohydrates may play a role in cholesterol gallstone formation by altering biliary motility and by enhancing crystal formation.

Original languageEnglish (US)
Pages (from-to)654-659
Number of pages6
JournalSurgery
Volume141
Issue number5
DOIs
StatePublished - May 1 2007

Fingerprint

Dietary Carbohydrates
Gallbladder
Cholesterol
Diet
Obese Mice
Carbohydrates
Bile
Observation
Gallstones
Cholecystectomy
Leptin
Baths

ASJC Scopus subject areas

  • Surgery

Cite this

Mathur, A., Megan, M., Al-Azzawi, H. H., Lu, D., Swartz-Basile, D. A., Nakeeb, A., & Pitt, H. A. (2007). High dietary carbohydrates decrease gallbladder volume and enhance cholesterol crystal formation. Surgery, 141(5), 654-659. https://doi.org/10.1016/j.surg.2006.11.008

High dietary carbohydrates decrease gallbladder volume and enhance cholesterol crystal formation. / Mathur, Abhishek; Megan, Marine; Al-Azzawi, Hayder H.; Lu, Debao; Swartz-Basile, Deborah A.; Nakeeb, Attila; Pitt, Henry A.

In: Surgery, Vol. 141, No. 5, 01.05.2007, p. 654-659.

Research output: Contribution to journalArticle

Mathur, A, Megan, M, Al-Azzawi, HH, Lu, D, Swartz-Basile, DA, Nakeeb, A & Pitt, HA 2007, 'High dietary carbohydrates decrease gallbladder volume and enhance cholesterol crystal formation', Surgery, vol. 141, no. 5, pp. 654-659. https://doi.org/10.1016/j.surg.2006.11.008
Mathur, Abhishek ; Megan, Marine ; Al-Azzawi, Hayder H. ; Lu, Debao ; Swartz-Basile, Deborah A. ; Nakeeb, Attila ; Pitt, Henry A. / High dietary carbohydrates decrease gallbladder volume and enhance cholesterol crystal formation. In: Surgery. 2007 ; Vol. 141, No. 5. pp. 654-659.
@article{b339427193cb4f588f222efdba513dc8,
title = "High dietary carbohydrates decrease gallbladder volume and enhance cholesterol crystal formation",
abstract = "Background: Animal and human data suggest that a diet high in refined carbohydrates leads to gallstone formation. However, no data are available on the role of dietary carbohydrates on gallbladder volume or on cholesterol crystal formation. Therefore, we tested the hypothesis that a high carbohydrate diet would alter gallbladder volume and enhance cholesterol crystal formation. Methods: At 8 weeks of age, 60 lean and 36 obese leptin-deficient female mice were fed a 45{\%} carbohydrate diet while an equal number of lean and obese mice were fed a 75{\%} carbohydrate diet for 4 weeks. All animals then underwent cholecystectomy, and gallbladder bile volume was recorded. Bile was pooled, filtered, and maintained in a water bath at 37°C for 14 days. Birefringent cholesterol crystals in bile were counted daily; crystal observation time and crystal mass were determined. Results: The crystal observation time was significantly shortened in both lean and obese mice on the 75{\%} diet compared with their counterparts on the 45{\%} diet. The crystal mass was significantly increased in the lean mice on the 75{\%} diet compared with the 45{\%} diet. Gallbladder volumes were significantly reduced in both lean and obese mice on the 75{\%} diet compared with their counterparts on the 45{\%} diet. Conclusions: These data suggest that a high carbohydrate diet decreases gallbladder volume, shortens cholesterol crystal observation time, and increases crystal mass. We conclude that dietary carbohydrates may play a role in cholesterol gallstone formation by altering biliary motility and by enhancing crystal formation.",
author = "Abhishek Mathur and Marine Megan and Al-Azzawi, {Hayder H.} and Debao Lu and Swartz-Basile, {Deborah A.} and Attila Nakeeb and Pitt, {Henry A.}",
year = "2007",
month = "5",
day = "1",
doi = "10.1016/j.surg.2006.11.008",
language = "English (US)",
volume = "141",
pages = "654--659",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - High dietary carbohydrates decrease gallbladder volume and enhance cholesterol crystal formation

AU - Mathur, Abhishek

AU - Megan, Marine

AU - Al-Azzawi, Hayder H.

AU - Lu, Debao

AU - Swartz-Basile, Deborah A.

AU - Nakeeb, Attila

AU - Pitt, Henry A.

PY - 2007/5/1

Y1 - 2007/5/1

N2 - Background: Animal and human data suggest that a diet high in refined carbohydrates leads to gallstone formation. However, no data are available on the role of dietary carbohydrates on gallbladder volume or on cholesterol crystal formation. Therefore, we tested the hypothesis that a high carbohydrate diet would alter gallbladder volume and enhance cholesterol crystal formation. Methods: At 8 weeks of age, 60 lean and 36 obese leptin-deficient female mice were fed a 45% carbohydrate diet while an equal number of lean and obese mice were fed a 75% carbohydrate diet for 4 weeks. All animals then underwent cholecystectomy, and gallbladder bile volume was recorded. Bile was pooled, filtered, and maintained in a water bath at 37°C for 14 days. Birefringent cholesterol crystals in bile were counted daily; crystal observation time and crystal mass were determined. Results: The crystal observation time was significantly shortened in both lean and obese mice on the 75% diet compared with their counterparts on the 45% diet. The crystal mass was significantly increased in the lean mice on the 75% diet compared with the 45% diet. Gallbladder volumes were significantly reduced in both lean and obese mice on the 75% diet compared with their counterparts on the 45% diet. Conclusions: These data suggest that a high carbohydrate diet decreases gallbladder volume, shortens cholesterol crystal observation time, and increases crystal mass. We conclude that dietary carbohydrates may play a role in cholesterol gallstone formation by altering biliary motility and by enhancing crystal formation.

AB - Background: Animal and human data suggest that a diet high in refined carbohydrates leads to gallstone formation. However, no data are available on the role of dietary carbohydrates on gallbladder volume or on cholesterol crystal formation. Therefore, we tested the hypothesis that a high carbohydrate diet would alter gallbladder volume and enhance cholesterol crystal formation. Methods: At 8 weeks of age, 60 lean and 36 obese leptin-deficient female mice were fed a 45% carbohydrate diet while an equal number of lean and obese mice were fed a 75% carbohydrate diet for 4 weeks. All animals then underwent cholecystectomy, and gallbladder bile volume was recorded. Bile was pooled, filtered, and maintained in a water bath at 37°C for 14 days. Birefringent cholesterol crystals in bile were counted daily; crystal observation time and crystal mass were determined. Results: The crystal observation time was significantly shortened in both lean and obese mice on the 75% diet compared with their counterparts on the 45% diet. The crystal mass was significantly increased in the lean mice on the 75% diet compared with the 45% diet. Gallbladder volumes were significantly reduced in both lean and obese mice on the 75% diet compared with their counterparts on the 45% diet. Conclusions: These data suggest that a high carbohydrate diet decreases gallbladder volume, shortens cholesterol crystal observation time, and increases crystal mass. We conclude that dietary carbohydrates may play a role in cholesterol gallstone formation by altering biliary motility and by enhancing crystal formation.

UR - http://www.scopus.com/inward/record.url?scp=34247261943&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247261943&partnerID=8YFLogxK

U2 - 10.1016/j.surg.2006.11.008

DO - 10.1016/j.surg.2006.11.008

M3 - Article

C2 - 17462466

AN - SCOPUS:34247261943

VL - 141

SP - 654

EP - 659

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 5

ER -