High-dose glucose induced apoptosis in human retinal vascular endothelial cells

H. C. Hatcher, M. B. Grant, S. Caballero, R. K. Crouch, J. X. Ma

Research output: Contribution to journalArticle

Abstract

Purpose: The purpose of this study is to determine the effect of high-dose glucose on primary cultured human retinal capillary endothelial cells and its potential involvement in retinal diseases such as diabetic retinopathy. Methods: Human retinal capillary endothelial cells were obtained from donor eyes. These cells were cultured in 5mM or 25mM glucose for 6 days prior to analysis. Transmission electron microscopy (TEM) was performed on the retinal endothelial cells to observe ultrastructural events in apoptosis. Flow cytometry using the propidium iodide exclusion assay was used to quantify apoptotic cells. DNA fragmentation was analyzed by electrophoresis in an agarose gel. Results: TEM revealed extensive cell surface blebbing with preservation of the cell membrane. Cell count revealed a significant decrease in viable cells (p1 peak in the high-dose glucose-treated cells which indicates the existence of a large number of apoptotic cells. Apoptosis was also supported by DNA fragmentation. Conclusions: High-dose glucose (25mM) induced cell death in human retinal capillary endothelial cells through the apoptosis pathway.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

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Retinal Vessels
Endothelial Cells
Apoptosis
Glucose
DNA Fragmentation
Transmission Electron Microscopy
Cell Count
Retinal Diseases
Agar Gel Electrophoresis
Propidium
Diabetic Retinopathy
Blister
Cultured Cells
Flow Cytometry
Cell Death
Cell Membrane

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Hatcher, H. C., Grant, M. B., Caballero, S., Crouch, R. K., & Ma, J. X. (1996). High-dose glucose induced apoptosis in human retinal vascular endothelial cells. Investigative Ophthalmology and Visual Science, 37(3).

High-dose glucose induced apoptosis in human retinal vascular endothelial cells. / Hatcher, H. C.; Grant, M. B.; Caballero, S.; Crouch, R. K.; Ma, J. X.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Hatcher, HC, Grant, MB, Caballero, S, Crouch, RK & Ma, JX 1996, 'High-dose glucose induced apoptosis in human retinal vascular endothelial cells', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Hatcher, H. C. ; Grant, M. B. ; Caballero, S. ; Crouch, R. K. ; Ma, J. X. / High-dose glucose induced apoptosis in human retinal vascular endothelial cells. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
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