High resolution 4-dimension imaging of metanephric embryonic kidney morphogenesis

Sherry G. Clendenon, Heather H. Ward, Kenneth W. Dunn, Robert Bacallao

Research output: Contribution to journalArticle

1 Scopus citations


High-resolution three-dimensional imaging of fixed embryonic kidney tissues has advanced considerably in the past decade. Here we developed a new process for imaging whole metanephric organ culture at cell resolution in three dimensions over time. This technique combines the use of the newly available generation of infrared-optimized long working distance, high numerical aperture objectives and multiphoton fluorescence microscopy with a new system for vital staining of metanephric organ cultures with bodipy ceramide. This allows all cells in the organ culture to be visualized over time, enabling detailed observation of tissue morphogenesis. Thus, our method offers a powerful new approach for visualizing and understanding early events in renal development and for extending observations made in genetically manipulated models.

Original languageEnglish (US)
Pages (from-to)757-761
Number of pages5
JournalKidney international
Issue number4
StatePublished - Apr 2013


  • kidney
  • metanephric organ culture
  • morphogenesis
  • two-photon

ASJC Scopus subject areas

  • Nephrology

Fingerprint Dive into the research topics of 'High resolution 4-dimension imaging of metanephric embryonic kidney morphogenesis'. Together they form a unique fingerprint.

  • Cite this