High-resolution high-speed synchronous epifluorescence imaging of cardiac activation

Shien-Fong Lin, Rashida A. Abbas, John P. Wikswo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

An optical imaging technique with high spatial and temporal resolution was developed to record fractional changes in laser-induced epifluorescence associated with the cardiac transmembrane potential during and after the application of monophasic point stimuli. The technique takes advantage of the repeatability of the recorded events, and uses a synchronized laser strobing mechanism to overcome the speed limitation inherent to slow-scan charge-coupled device cameras, and achieves an effective frame rate of 500 frames/s at a spatial resolution of 100×100 pixels in a single frame with a pixel resolution of 75 μm. The signal-to-noise ratio can be improved with boxcar averaging. Patterns of virtual cathode and anode with distinctive regions of simultaneous depolarization and hyperpolarization during stimulation are demonstrated with stimuli applied to the resting myocardium of an isolated rabbit heart. The technique described in this article provides a powerful tool for investigating repeatable dynamics in the function of electrically active tissue.

Original languageEnglish (US)
Pages (from-to)213-217
Number of pages5
JournalReview of Scientific Instruments
Volume68
Issue number1
StatePublished - Jan 1997
Externally publishedYes

Fingerprint

Pixels
Chemical activation
high speed
activation
Imaging techniques
stimuli
Lasers
high resolution
Depolarization
CCD cameras
spatial resolution
pixels
myocardium
Signal to noise ratio
Anodes
Cathodes
rabbits
Tissue
temporal resolution
stimulation

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)
  • Instrumentation

Cite this

High-resolution high-speed synchronous epifluorescence imaging of cardiac activation. / Lin, Shien-Fong; Abbas, Rashida A.; Wikswo, John P.

In: Review of Scientific Instruments, Vol. 68, No. 1, 01.1997, p. 213-217.

Research output: Contribution to journalArticle

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