High-resolution X-ray structure of an acyl-enzyme species for the class D OXA-10, β-lactamase

Laurent Maveyraud, Dasantila Golemi-Kotra, Akihiro Ishiwata, Samy Meroueh, Shahriar Mobashery, Jean Pierre Samama

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

β-Lactamases are resistance enzymes for β-lactam antibiotics, These enzymes hydrolyze the β-lactam moieties of these antibiotics, rendering them inactive. Of the four classes of known β-lactamases, the enzymes of class D are the least understood. We report herein the high-resolution (1.9 Å) crystal structure of the class D OXA-10 β-lactamase inhibited by a penicillanate derivative. The structure provides evidence that the carboxylated Lys-70 (a carbamate) is intimately involved in the mechanism of the enzyme.

Original languageEnglish (US)
Pages (from-to)2461-2465
Number of pages5
JournalJournal of the American Chemical Society
Volume124
Issue number11
DOIs
StatePublished - Mar 20 2002
Externally publishedYes

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Lactams
Enzymes
X-Rays
4 alpha-glucanotransferase
X rays
Antibiotics
Anti-Bacterial Agents
Carbamates
Crystal structure
Derivatives
beta-lactamase PSE-2

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

High-resolution X-ray structure of an acyl-enzyme species for the class D OXA-10, β-lactamase. / Maveyraud, Laurent; Golemi-Kotra, Dasantila; Ishiwata, Akihiro; Meroueh, Samy; Mobashery, Shahriar; Samama, Jean Pierre.

In: Journal of the American Chemical Society, Vol. 124, No. 11, 20.03.2002, p. 2461-2465.

Research output: Contribution to journalArticle

Maveyraud, Laurent ; Golemi-Kotra, Dasantila ; Ishiwata, Akihiro ; Meroueh, Samy ; Mobashery, Shahriar ; Samama, Jean Pierre. / High-resolution X-ray structure of an acyl-enzyme species for the class D OXA-10, β-lactamase. In: Journal of the American Chemical Society. 2002 ; Vol. 124, No. 11. pp. 2461-2465.
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