High-resolution X-ray structure of an acyl-enzyme species for the class D OXA-10, β-lactamase

Laurent Maveyraud, Dasantila Golemi-Kotra, Akihiro Ishiwata, Oussama Meroueh, Shahriar Mobashery, Jean Pierre Samama

Research output: Contribution to journalArticle

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Abstract

β-Lactamases are resistance enzymes for β-lactam antibiotics, These enzymes hydrolyze the β-lactam moieties of these antibiotics, rendering them inactive. Of the four classes of known β-lactamases, the enzymes of class D are the least understood. We report herein the high-resolution (1.9 Å) crystal structure of the class D OXA-10 β-lactamase inhibited by a penicillanate derivative. The structure provides evidence that the carboxylated Lys-70 (a carbamate) is intimately involved in the mechanism of the enzyme.

Original languageEnglish (US)
Pages (from-to)2461-2465
Number of pages5
JournalJournal of the American Chemical Society
Volume124
Issue number11
DOIs
StatePublished - Mar 20 2002

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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