β-Lactamases are resistance enzymes for β-lactam antibiotics, These enzymes hydrolyze the β-lactam moieties of these antibiotics, rendering them inactive. Of the four classes of known β-lactamases, the enzymes of class D are the least understood. We report herein the high-resolution (1.9 Å) crystal structure of the class D OXA-10 β-lactamase inhibited by a penicillanate derivative. The structure provides evidence that the carboxylated Lys-70 (a carbamate) is intimately involved in the mechanism of the enzyme.
ASJC Scopus subject areas
- Colloid and Surface Chemistry