High-throughput acoustofluidic fabrication of tumor spheroids

Bin Chen, Yue Wu, Zheng Ao, Hongwei Cai, Asael Nunez, Yunhua Liu, John Foley, Kenneth Nephew, Xiongbin Lu, Feng Guo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Three-dimensional (3D) culture of multicellular spheroids, offering a desirable biomimetic microenvironment, is appropriate for recapitulating tissue cellular adhesive complexity and revealing a more realistic drug response. However, current 3D culture methods are suffering from low-throughput, poor controllability, intensive-labor, and variation in spheroid size, thus not ready for many high-throughput screening applications including drug discovery and toxicity testing. Herein, we developed a high-throughput multicellular spheroid fabrication method using acoustofluidics. By acoustically-assembling cancer cells with low-cost and disposable devices, our method can produce more than 12000 multicellular aggregates within several minutes and allow us to transfer these aggregates into ultra-low attachment dishes for long-term culture. This method can generate more than 6000 tumor spheroids per operation, and reduce tumor spheroid formation time to one day. Our platform has advantages in forming spheroids with high throughput, short time, and long-term effectiveness, and is easy-to-operation. This acoustofluidic spheroid assembly method provides a simple and efficient way to produce large numbers of uniform-sized spheroids for biomedical applications in translational medicine, pharmaceutical industry and basic life science research.

Original languageEnglish (US)
Pages (from-to)1755-1763
Number of pages9
JournalLab on a Chip
Volume19
Issue number10
DOIs
StatePublished - Jan 1 2019

Fingerprint

Tumors
Throughput
Fabrication
Cellular Spheroids
Neoplasms
Tissue Adhesives
Biomimetics
Controllability
Translational Medical Research
Pharmaceutical Preparations
Drug products
Biological Science Disciplines
Medicine
Toxicity
Drug Industry
Drug Discovery
Adhesives
Screening
Drug-Related Side Effects and Adverse Reactions
Cells

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Chen, B., Wu, Y., Ao, Z., Cai, H., Nunez, A., Liu, Y., ... Guo, F. (2019). High-throughput acoustofluidic fabrication of tumor spheroids. Lab on a Chip, 19(10), 1755-1763. https://doi.org/10.1039/c9lc00135b

High-throughput acoustofluidic fabrication of tumor spheroids. / Chen, Bin; Wu, Yue; Ao, Zheng; Cai, Hongwei; Nunez, Asael; Liu, Yunhua; Foley, John; Nephew, Kenneth; Lu, Xiongbin; Guo, Feng.

In: Lab on a Chip, Vol. 19, No. 10, 01.01.2019, p. 1755-1763.

Research output: Contribution to journalArticle

Chen, B, Wu, Y, Ao, Z, Cai, H, Nunez, A, Liu, Y, Foley, J, Nephew, K, Lu, X & Guo, F 2019, 'High-throughput acoustofluidic fabrication of tumor spheroids', Lab on a Chip, vol. 19, no. 10, pp. 1755-1763. https://doi.org/10.1039/c9lc00135b
Chen B, Wu Y, Ao Z, Cai H, Nunez A, Liu Y et al. High-throughput acoustofluidic fabrication of tumor spheroids. Lab on a Chip. 2019 Jan 1;19(10):1755-1763. https://doi.org/10.1039/c9lc00135b
Chen, Bin ; Wu, Yue ; Ao, Zheng ; Cai, Hongwei ; Nunez, Asael ; Liu, Yunhua ; Foley, John ; Nephew, Kenneth ; Lu, Xiongbin ; Guo, Feng. / High-throughput acoustofluidic fabrication of tumor spheroids. In: Lab on a Chip. 2019 ; Vol. 19, No. 10. pp. 1755-1763.
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