High-throughput immunomagnetic cell detection using a microaperture chip system

Chun Li Chang, Shadia I. Jalal, Wanfeng Huang, Aamer Mahmood, Daniela E. Matei, Cagri A. Savran

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We report a microchip system based on a combination of immunomagnetic separation, microfluidics, and size-based filtration for high-throughput detection of rare cells. In this system, target cells bind to magnetic beads in vitro and flow parallel to a microchip with flow rates of milliliters/minute. A magnetic field draws the bead-bound cells toward the microchip, which contains apertures that allow passage of unbound beads while trapping the target cells. The cells captured on the chip can be investigated clearly under a microscope and released from the chip for further analysis. We first characterize the system by detecting cancer cell lines (MCF-7 and A549) in culture media. We then demonstrate detection of 100 MCF-7 cells spiked in 7.5 mL of human blood to simulate detection of circulating tumor cells present in cancer patient blood samples. On average, 85% of the spiked cells were detected. We expect this system to be highly useful in a wide variety of clinical as well as other applications that seek rare cells.

Original languageEnglish (US)
Article number6808412
Pages (from-to)3008-3013
Number of pages6
JournalIEEE Sensors Journal
Volume14
Issue number9
DOIs
StatePublished - Sep 2014

Keywords

  • CTC detection
  • immunomagnetic separation
  • microfluidics
  • rare cell detection
  • size-based filtration
  • tumor cells

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Instrumentation

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