Highly specific plasmonic biosensors for ultrasensitive MicroRNA detection in plasma from pancreatic cancer patients

Gayatri K. Joshi, Samantha Deitz-Mcelyea, Merrell Johnson, Sonali Mali, Murray Korc, Rajesh Sardar

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

MicroRNAs (miRs) are small noncoding RNAs that regulate mRNA stability and/or translation. Because of their release into the circulation and their remarkable stability, miR levels in plasma and other biological fluids can serve as diagnostic and prognostic disease biomarkers. However, quantifying miRs in the circulation is challenging due to issues with sensitivity and specificity. This Letter describes for the first time the design and characterization of a regenerative, solid-state localized surface plasmon resonance (LSPR) sensor based on highly sensitive nanostructures (gold nanoprisms) that obviates the need for labels or amplification of the miRs. Our direct hybridization approach has enabled the detection of subfemtomolar concentration of miR-X (X = 21 and 10b) in human plasma in pancreatic cancer patients. Our LSPR-based measurements showed that the miR levels measured directly in patient plasma were at least 2-fold higher than following RNA extraction and quantification by reverse transcriptase-polymerase chain reaction. Through LSPR-based measurements we have shown nearly 4-fold higher concentrations of miR-10b than miR-21 in plasma of pancreatic cancer patients. We propose that our highly sensitive and selective detection approach for assaying miRs in plasma can be applied to many cancer types and disease states and should allow a rational approach for testing the utility of miRs as markers for early disease diagnosis and prognosis, which could allow for the design of effective individualized therapeutic approaches.

Original languageEnglish
Pages (from-to)6955-6963
Number of pages9
JournalNano Letters
Volume14
Issue number12
DOIs
StatePublished - Dec 10 2014

Fingerprint

MicroRNAs
bioinstrumentation
Biosensors
Surface plasmon resonance
cancer
Plasmas
surface plasmon resonance
RNA
Plasma (human)
assaying
Polymerase chain reaction
Biomarkers
Small Untranslated RNA
polymerase chain reaction
prognosis
biomarkers
RNA-Directed DNA Polymerase
Amplification
Labels
Nanostructures

Keywords

  • label-free detection
  • localized surface plasmon resonance
  • microRNAs
  • Nanoprisms
  • pancreatic cancer
  • patient plasma

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Joshi, G. K., Deitz-Mcelyea, S., Johnson, M., Mali, S., Korc, M., & Sardar, R. (2014). Highly specific plasmonic biosensors for ultrasensitive MicroRNA detection in plasma from pancreatic cancer patients. Nano Letters, 14(12), 6955-6963. https://doi.org/10.1021/nl503220s

Highly specific plasmonic biosensors for ultrasensitive MicroRNA detection in plasma from pancreatic cancer patients. / Joshi, Gayatri K.; Deitz-Mcelyea, Samantha; Johnson, Merrell; Mali, Sonali; Korc, Murray; Sardar, Rajesh.

In: Nano Letters, Vol. 14, No. 12, 10.12.2014, p. 6955-6963.

Research output: Contribution to journalArticle

Joshi, GK, Deitz-Mcelyea, S, Johnson, M, Mali, S, Korc, M & Sardar, R 2014, 'Highly specific plasmonic biosensors for ultrasensitive MicroRNA detection in plasma from pancreatic cancer patients', Nano Letters, vol. 14, no. 12, pp. 6955-6963. https://doi.org/10.1021/nl503220s
Joshi, Gayatri K. ; Deitz-Mcelyea, Samantha ; Johnson, Merrell ; Mali, Sonali ; Korc, Murray ; Sardar, Rajesh. / Highly specific plasmonic biosensors for ultrasensitive MicroRNA detection in plasma from pancreatic cancer patients. In: Nano Letters. 2014 ; Vol. 14, No. 12. pp. 6955-6963.
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