Histologic findings after in vivo placement of small intestine submucosal vascular grafts and saphenous vein grafts in the carotid artery in dogs

George Sandusky, S. F. Badylak, R. J. Morff, W. D. Johnson, G. Lantz

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Abstract

A small caliber vascular graft from porcine small intestine submucosa (SIS) was implanted in a canine carotid artery (n = 24) and compared with an autogenous saphenous vein graft that was implanted in the contralateral carotid artery. In this study, four grafts were evaluated at the following times after surgery: 2, 7, 14, 28, 90, and 180 days. One SIS graft thrombosed at 2 days, two SIS and two saphenous vein grafts were thrombosed at 90 days, and one SIS and one saphenous vein graft were thrombosed at 180 days. At 2 days after implant, the luminal surface of the SIS graft was covered by a thin (30 μ) fibrin meshwork. By 14 days after surgery, endothelial cells on the fibrin meshwork were staining for FVIII-related antigen. Smooth muscle cells were observed in the new intima (fibrin meshwork) by 28 days. At 90 days, both types of graft had arterialized with an intima covered by endothelium, a smooth muscle media, and marked adventitial fibrosis. Similar histology was observed at 180 days. These results indicate that this SIS graft was similar to saphenous vein graft in the dog.

Original languageEnglish (US)
Pages (from-to)317-324
Number of pages8
JournalAmerican Journal of Pathology
Volume140
Issue number2
StatePublished - Feb 1992
Externally publishedYes

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Saphenous Vein
Carotid Arteries
Small Intestine
Blood Vessels
Dogs
Transplants
Fibrin
Thrombosis
Adventitia
Ambulatory Surgical Procedures
Smooth Muscle Myocytes
Endothelium
Smooth Muscle
Canidae
Histology
Fibrosis
Swine
Endothelial Cells
Staining and Labeling
Antigens

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Histologic findings after in vivo placement of small intestine submucosal vascular grafts and saphenous vein grafts in the carotid artery in dogs. / Sandusky, George; Badylak, S. F.; Morff, R. J.; Johnson, W. D.; Lantz, G.

In: American Journal of Pathology, Vol. 140, No. 2, 02.1992, p. 317-324.

Research output: Contribution to journalArticle

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