Histological Effects of Procedural and Environmental Factors on Isolated Rat Heart Preparations

John W. Brown, Dennis Cristian, Raymond R. Paradise

Research output: Contribution to journalArticle

7 Scopus citations


Histological evaluation of isolated rat heart preparations has shown that: Incubated isolated right ventricular strips and atria undergo observable degeneration after relatively short periods of time and at temperatures of 27° or higher. The degree of degeneration is a function of: (a) duration of incubation; (b) incubation temperature: and (c) thickness of the soaked preparation. Cardiac degeneration is not prevented by penicillin, streptomycin, polymyxin, chlorte-tracycline, 9-alpha-fluorohydrocortisone, or ouabain in the doses employed. The perfused whole heart is superior to the incubated isolated tissue preparation. The perfused heart shows few signs of degeneration at 37° for prolonged periods.

Original languageEnglish (US)
Pages (from-to)455-462
Number of pages8
JournalProceedings of the Society for Experimental Biology and Medicine
Issue number2
StatePublished - Nov 1968


ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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