Histological validation of dopamine transporter PET images in the rat 6-OHDA lesion model of Parkinson's disease

Bruce D. Gitter, Jeffrey W. Horn, Jessica M. Brown, Paul R. Territo, Wendy Winkle, Karmen K. Yoder, Gary D. Hutchins, Diane L. Sterchi

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Positron emission tomography (PET) imaging of dopamine reuptake transporters (DAT) in the rat 6-hydoxydopamine (6-OHDA) lesion model of Parkinson's disease (PD) is a clinically translatable biomarker that could be used in the discovery of novel, disease-modifying PD therapeutics. Careful histological validation of DAT PET images are critical steps in overcoming clinical imaging artifacts seen in PD patients undergoing dopamine replacement therapy. In this report, we describe the use of quantitative DAT autoradiography and tyrosine hydroxylase immunohistochemistry methods using adjacent cryosections to provide histological validation of DAT PET images and verify the loss of DAT binding sites with reduced survival of dopaminergic neurons in the nigrostriatal pathway.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalJournal of Histotechnology
Volume30
Issue number2
DOIs
StatePublished - Jun 2007

Keywords

  • Dopamine reuptake transporters (DAT)
  • Positron emission tomography (PET)
  • Quantitative receptor autoradiography
  • Tyrosine hydroxylase immunohistochemistry

ASJC Scopus subject areas

  • Histology
  • Anatomy
  • Medical Laboratory Technology

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