Histological validation of dopamine transporter PET images in the rat 6-OHDA lesion model of Parkinson's disease

Bruce D. Gitter, Jeffrey W. Horn, Jessica M. Brown, Paul Territo, Wendy Winkle, Karmen Yoder, Gary Hutchins, Diane L. Sterchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Positron emission tomography (PET) imaging of dopamine reuptake transporters (DAT) in the rat 6-hydoxydopamine (6-OHDA) lesion model of Parkinson's disease (PD) is a clinically translatable biomarker that could be used in the discovery of novel, disease-modifying PD therapeutics. Careful histological validation of DAT PET images are critical steps in overcoming clinical imaging artifacts seen in PD patients undergoing dopamine replacement therapy. In this report, we describe the use of quantitative DAT autoradiography and tyrosine hydroxylase immunohistochemistry methods using adjacent cryosections to provide histological validation of DAT PET images and verify the loss of DAT binding sites with reduced survival of dopaminergic neurons in the nigrostriatal pathway.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalJournal of Histotechnology
Volume30
Issue number2
StatePublished - Jun 2007

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Dopamine Plasma Membrane Transport Proteins
Oxidopamine
Positron-Emission Tomography
Parkinson Disease
Dopaminergic Neurons
Tyrosine 3-Monooxygenase
Autoradiography
Artifacts
Dopamine
Biomarkers
Immunohistochemistry
Binding Sites
Therapeutics

Keywords

  • Dopamine reuptake transporters (DAT)
  • Positron emission tomography (PET)
  • Quantitative receptor autoradiography
  • Tyrosine hydroxylase immunohistochemistry

ASJC Scopus subject areas

  • Histology
  • Anatomy
  • Medical Laboratory Technology

Cite this

Histological validation of dopamine transporter PET images in the rat 6-OHDA lesion model of Parkinson's disease. / Gitter, Bruce D.; Horn, Jeffrey W.; Brown, Jessica M.; Territo, Paul; Winkle, Wendy; Yoder, Karmen; Hutchins, Gary; Sterchi, Diane L.

In: Journal of Histotechnology, Vol. 30, No. 2, 06.2007, p. 123-129.

Research output: Contribution to journalArticle

Gitter, Bruce D. ; Horn, Jeffrey W. ; Brown, Jessica M. ; Territo, Paul ; Winkle, Wendy ; Yoder, Karmen ; Hutchins, Gary ; Sterchi, Diane L. / Histological validation of dopamine transporter PET images in the rat 6-OHDA lesion model of Parkinson's disease. In: Journal of Histotechnology. 2007 ; Vol. 30, No. 2. pp. 123-129.
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