Histology-specific expression of a dna repair protein in pediatric rhabdomyosarcomas

Blythe Thomson, Renee Tritt, Mary Davis, Mark R. Kelley

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Purpose: DNA repair enzymes have a critical role in cellular maintenance and survival. The enzyme apurinic/apyrimidinic endonuclease/redox factor 1 (APE/ref1), a key protein in the base excision repair pathway, displays both repair and redox control. We examined the role of APE/ref1 in pediatric embryonal and alveolar rhabdomyosarcomas (ARMS). Materials and Methods: Using an immunohistochemical method, fixed tissue from 31 newly diagnosed pediatric rhabdomyosarcomas were evaluated for expression of APE/ref1. Tissue was obtained from Indiana University and the Cooperative Human Tissue Network. Results: We demonstrated high levels of expression within the localized and metastatic embryonal rhabdomyosarcomas. This contrasted with both localized and metastatic ARMS, which had low levels of APE/ref1 expression. This histology-specific difference proved to be significant (P = 0.003). Furthermore, the expression within all tumors examined was localized to the nucleus and did not differ between localized and metastatic tumors. Conclusions: We propose several hypotheses to explain this histology-specific expression of APE/ref1 in pediatric rhabdomyosarcomas. Because the majority of ARMS expressed either the PAX3/FKHR or PAX7/FKHR fusion transcript, the low level of expression may be related to the redox activity of APE/ref1. The low levels may also be related to the bioreductive activity of APE/ref1.

Original languageEnglish (US)
Pages (from-to)234-239
Number of pages6
JournalAmerican Journal of Pediatric Hematology/Oncology
Volume23
Issue number4
DOIs
StatePublished - Jan 1 2001

Keywords

  • DNA repair
  • Redox
  • Rhabdomyosarcomas

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Oncology
  • Hematology

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