Histology-specific expression of a DNA repair protein in pediatric rhabdomyosarcomas

B. Thomson, R. Tritt, M. Davis, Mark Kelley

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Purpose: DNA repair enzymes have a critical role in cellular maintenance and survival. The enzyme apurinic/apyrimidinic endonuclease/redox factor 1 (APE/ref1), a key protein in the base excision repair pathway, displays both repair and redox control. We examined the role of APE/ref1 in pediatric embryonal and alveolar rhabdomyosarcomas (ARMS). Materials and Methods: Using an immunohistochemical method, fixed tissue from 31 newly diagnosed pediatric rhabdomyosarcomas were evaluated for expression of APE/ref1. Tissue was obtained from Indiana University and the Cooperative Human Tissue Network. Results: We demonstrated high levels of expression within the localized and metastatic embryonal rhabdomyosarcomas. This contrasted with both localized and metastatic ARMS, which had low levels of APE/ref1 expression. This histology-specific difference proved to be significant (P = 0.003). Furthermore, the expression within all tumors examined was localized to the nucleus and did not differ between localized and metastatic tumors. Conclusions: We propose several hypotheses to explain this histology-specific expression of APE/ref1 in pediatric rhabdomyosarcomas. Because the majority of ARMS expressed either the PAX3/FKHR or PAX7/FKHR fusion transcript, the low level of expression may be related to the redox activity of APE/ref1. The low levels may also be related to the bioreductive activity of APE/ref1.

Original languageEnglish
Pages (from-to)234-239
Number of pages6
JournalJournal of Pediatric Hematology/Oncology
Volume23
Issue number4
DOIs
StatePublished - 2001

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Rhabdomyosarcoma
DNA Repair
Oxidation-Reduction
Histology
Endonucleases
Pediatrics
Alveolar Rhabdomyosarcoma
Proteins
Embryonal Rhabdomyosarcoma
DNA-(Apurinic or Apyrimidinic Site) Lyase
DNA Repair Enzymes
Neoplasms
Maintenance
Survival
Enzymes

Keywords

  • DNA repair
  • Redox
  • Rhabdomyosarcomas

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Oncology
  • Hematology

Cite this

Histology-specific expression of a DNA repair protein in pediatric rhabdomyosarcomas. / Thomson, B.; Tritt, R.; Davis, M.; Kelley, Mark.

In: Journal of Pediatric Hematology/Oncology, Vol. 23, No. 4, 2001, p. 234-239.

Research output: Contribution to journalArticle

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