Histone H4 HDAC activity is necessary for expression of the PU.1 gene

R. Nicholas Laribee, Michael J. Klemsz

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Although the current paradigm delegates histone deacetylases (HDACs) to the role of transcriptional co-repressors, we recently showed that HDAC activity was necessary for expression of the hematopoietic transcription factor PU.1. Chromatin immunoprecipitation analyses showed that inhibition of HDACs resulted in increased histone H4 acetylation within the promoter and intron 1 regions of the PU.1 locus. In contrast, increases in both H3 and H4 acetylation were seen for introns 2, 3 and 4 on the 3′ end of the PU.1 locus. Maximal increases in histone H4 acetylation over the promoter and intron 1 region were seen within 10 min of HDAC inhibition, while the increases seen on the 3′ end showed slower kinetics. The increases in H4 acetylation were reversible and decreased levels of acetylation correlated with re-expression of the PU.1 gene. Finally, we show that HDAC activity is required for association of RNA polymerase II with the PU.1 promoter.

Original languageEnglish (US)
Pages (from-to)226-234
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1730
Issue number3
DOIs
StatePublished - Sep 25 2005

Keywords

  • Acetylation
  • Histone deacetylase
  • Inhibition

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

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