Hormonal regulation and expression of the jun-D protooncogene in specific cell types of the rat uterus

Kenneth Nephew, David K. Webb, Kamil Can Akcali, Bruce C. Moulton, Sohaib A. Khan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Steroid hormone regulation and cell-type specific expression of the jun-D protooncogene in rat uterus was examined. Adult, ovariectomized rats were injected with progesterone, testosterone, 17β-estradiol (E2-17β), 16α-estradiol (E2-16α), dexamethasone or cycloheximide. Uteri were collected between 0 and 6 h post-treatment. Northern blot analysis of uterine RNA revealed that induction of jun-D was specific for estrogenic steroids, as progesterone and testosterone had no effect on expression of this member of the jun gene family. Treatment with E2-17β increased jun-D mRNA levels by approx. 5-fold, with expression reaching peak levels at 3 h after treatment and declining thereafter. Administration of E2-16α, a short-acting estrogen that does not cause uterine cell proliferation, increased expression of jun-D but with different kinetics compared to the long-acting E2-17β. The mRNA levels of jun-D increased by 3-fold 1 h after administration of E2-16α but declined soon after. Slight induction of jun-D mRNA by dexamethasone was apparent, but to a much lesser extent compared to estrogen. The protein synthesis inhibitor, cycloheximide, did not block jun-D induction, indicating that this is an "immediate early" response. Expression of Jun-D protein was examined by immunohistochemical methods. E2-17β treatment activated jun-D primarily in the nuclei of luminal and glandular epithelial cells of the endometrium. These results demonstrate that hormonal induction of jun-D is specific for estrogens and that uterine expression of this protooncogene occurs in a cell-type specific manner.

Original languageEnglish (US)
Pages (from-to)281-287
Number of pages7
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume46
Issue number3
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Uterus
Rats
Estrogens
Cycloheximide
Messenger RNA
Dexamethasone
Progesterone
Testosterone
Steroid hormones
Steroids
Proto-Oncogene Proteins c-jun
jun Genes
Protein Synthesis Inhibitors
Cell proliferation
Endometrium
Northern Blotting
Estradiol
Epithelial Cells
Cell Proliferation
Hormones

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Hormonal regulation and expression of the jun-D protooncogene in specific cell types of the rat uterus. / Nephew, Kenneth; Webb, David K.; Akcali, Kamil Can; Moulton, Bruce C.; Khan, Sohaib A.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 46, No. 3, 1993, p. 281-287.

Research output: Contribution to journalArticle

Nephew, Kenneth ; Webb, David K. ; Akcali, Kamil Can ; Moulton, Bruce C. ; Khan, Sohaib A. / Hormonal regulation and expression of the jun-D protooncogene in specific cell types of the rat uterus. In: Journal of Steroid Biochemistry and Molecular Biology. 1993 ; Vol. 46, No. 3. pp. 281-287.
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