Hormonal Regulation of Insulin-Like Growth Factor Binding Proteins and Insulin-Like Growth Factor I (IGF-I) Secretion in Porcine Stromal-Vascular Cultures

Xuening (Neal) Chen, G. J. Hausman, J. T. Wright

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21 Citations (Scopus)

Abstract

We examined the time course of insulin-like growth factor binding protein (IGFBP) secretion and hormonal regulation of IGFBP and IGF-I secretion in porcine stromal vascular (S-V) cultures. Primary cultures of S-V cells derived from porcine adipose tissue were exposed to serum-free media with or without hormone treatment. Time course studies indicated that secretion of four IGFBP (IGFBP-1, -2, - 3 and -4) by porcine S-V cells increased with time during the first 3 d after the switch to serum-free conditions and then decreased gradually. Growth hormone treatment stimulated secretion of each IGFBP by 47 ± 4.7, 46 ± 10.2, 70 ± 13.2, and 49 ± 5.6%, respectively, over control levels. The secretion of IGFBP-1, -2, -3, and -4 was enhanced by 91 ± 18, 80 ± 16, 74 ± 12, and 263 ± 48%, respectively, in T4-treated S-V cultures compared with untreated cultures. In contrast, dexamethasone reduced the abundance of the IGFBP by 28 to 50% of control levels. Insulin-like growth factor I secretion (844.63 ± 35.98 pg/mL) in vitro (3 d conditioned media) was increased (P <.05) by GH (1,302.45 ± 12.95 pg/mL) and T4 (1,291.60 ± 86.4 pg/mL) and decreased (P <.05) by dexamethasone (552.5 ± 30.2 pg/mL) (n = 4, S-V cell pools, P <.05). In addition to preadipocytes, other cells in S-V cultures also secrete IGFBP. In conclusion, the secretion of IGF-I and IGFBP by S-V cells is differentially regulated by hormones in vitro.

Original languageEnglish (US)
Pages (from-to)2369-2375
Number of pages7
JournalJournal of Animal Science
Volume74
Issue number10
StatePublished - Oct 1996
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor Binding Proteins
insulin-like growth factor binding proteins
hormonal regulation
insulin-like growth factor I
Insulin-Like Growth Factor I
blood vessels
Blood Vessels
Swine
Stromal Cells
secretion
swine
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 1
Dexamethasone
L-thyroxine
dexamethasone
Hormones
hormones
Serum-Free Culture Media
cells

Keywords

  • Adipocytes
  • Binding Proteins
  • Hormones
  • Insulin-Like Growth Factor I
  • Regulation

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

@article{790988669f5243938a5cdf171b6a853b,
title = "Hormonal Regulation of Insulin-Like Growth Factor Binding Proteins and Insulin-Like Growth Factor I (IGF-I) Secretion in Porcine Stromal-Vascular Cultures",
abstract = "We examined the time course of insulin-like growth factor binding protein (IGFBP) secretion and hormonal regulation of IGFBP and IGF-I secretion in porcine stromal vascular (S-V) cultures. Primary cultures of S-V cells derived from porcine adipose tissue were exposed to serum-free media with or without hormone treatment. Time course studies indicated that secretion of four IGFBP (IGFBP-1, -2, - 3 and -4) by porcine S-V cells increased with time during the first 3 d after the switch to serum-free conditions and then decreased gradually. Growth hormone treatment stimulated secretion of each IGFBP by 47 ± 4.7, 46 ± 10.2, 70 ± 13.2, and 49 ± 5.6{\%}, respectively, over control levels. The secretion of IGFBP-1, -2, -3, and -4 was enhanced by 91 ± 18, 80 ± 16, 74 ± 12, and 263 ± 48{\%}, respectively, in T4-treated S-V cultures compared with untreated cultures. In contrast, dexamethasone reduced the abundance of the IGFBP by 28 to 50{\%} of control levels. Insulin-like growth factor I secretion (844.63 ± 35.98 pg/mL) in vitro (3 d conditioned media) was increased (P <.05) by GH (1,302.45 ± 12.95 pg/mL) and T4 (1,291.60 ± 86.4 pg/mL) and decreased (P <.05) by dexamethasone (552.5 ± 30.2 pg/mL) (n = 4, S-V cell pools, P <.05). In addition to preadipocytes, other cells in S-V cultures also secrete IGFBP. In conclusion, the secretion of IGF-I and IGFBP by S-V cells is differentially regulated by hormones in vitro.",
keywords = "Adipocytes, Binding Proteins, Hormones, Insulin-Like Growth Factor I, Regulation",
author = "Chen, {Xuening (Neal)} and Hausman, {G. J.} and Wright, {J. T.}",
year = "1996",
month = "10",
language = "English (US)",
volume = "74",
pages = "2369--2375",
journal = "Journal of Animal Science",
issn = "0021-8812",
publisher = "American Society of Animal Science",
number = "10",

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T1 - Hormonal Regulation of Insulin-Like Growth Factor Binding Proteins and Insulin-Like Growth Factor I (IGF-I) Secretion in Porcine Stromal-Vascular Cultures

AU - Chen, Xuening (Neal)

AU - Hausman, G. J.

AU - Wright, J. T.

PY - 1996/10

Y1 - 1996/10

N2 - We examined the time course of insulin-like growth factor binding protein (IGFBP) secretion and hormonal regulation of IGFBP and IGF-I secretion in porcine stromal vascular (S-V) cultures. Primary cultures of S-V cells derived from porcine adipose tissue were exposed to serum-free media with or without hormone treatment. Time course studies indicated that secretion of four IGFBP (IGFBP-1, -2, - 3 and -4) by porcine S-V cells increased with time during the first 3 d after the switch to serum-free conditions and then decreased gradually. Growth hormone treatment stimulated secretion of each IGFBP by 47 ± 4.7, 46 ± 10.2, 70 ± 13.2, and 49 ± 5.6%, respectively, over control levels. The secretion of IGFBP-1, -2, -3, and -4 was enhanced by 91 ± 18, 80 ± 16, 74 ± 12, and 263 ± 48%, respectively, in T4-treated S-V cultures compared with untreated cultures. In contrast, dexamethasone reduced the abundance of the IGFBP by 28 to 50% of control levels. Insulin-like growth factor I secretion (844.63 ± 35.98 pg/mL) in vitro (3 d conditioned media) was increased (P <.05) by GH (1,302.45 ± 12.95 pg/mL) and T4 (1,291.60 ± 86.4 pg/mL) and decreased (P <.05) by dexamethasone (552.5 ± 30.2 pg/mL) (n = 4, S-V cell pools, P <.05). In addition to preadipocytes, other cells in S-V cultures also secrete IGFBP. In conclusion, the secretion of IGF-I and IGFBP by S-V cells is differentially regulated by hormones in vitro.

AB - We examined the time course of insulin-like growth factor binding protein (IGFBP) secretion and hormonal regulation of IGFBP and IGF-I secretion in porcine stromal vascular (S-V) cultures. Primary cultures of S-V cells derived from porcine adipose tissue were exposed to serum-free media with or without hormone treatment. Time course studies indicated that secretion of four IGFBP (IGFBP-1, -2, - 3 and -4) by porcine S-V cells increased with time during the first 3 d after the switch to serum-free conditions and then decreased gradually. Growth hormone treatment stimulated secretion of each IGFBP by 47 ± 4.7, 46 ± 10.2, 70 ± 13.2, and 49 ± 5.6%, respectively, over control levels. The secretion of IGFBP-1, -2, -3, and -4 was enhanced by 91 ± 18, 80 ± 16, 74 ± 12, and 263 ± 48%, respectively, in T4-treated S-V cultures compared with untreated cultures. In contrast, dexamethasone reduced the abundance of the IGFBP by 28 to 50% of control levels. Insulin-like growth factor I secretion (844.63 ± 35.98 pg/mL) in vitro (3 d conditioned media) was increased (P <.05) by GH (1,302.45 ± 12.95 pg/mL) and T4 (1,291.60 ± 86.4 pg/mL) and decreased (P <.05) by dexamethasone (552.5 ± 30.2 pg/mL) (n = 4, S-V cell pools, P <.05). In addition to preadipocytes, other cells in S-V cultures also secrete IGFBP. In conclusion, the secretion of IGF-I and IGFBP by S-V cells is differentially regulated by hormones in vitro.

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