Hormonal Regulation of Insulin-Like Growth Factor Binding Proteins and Insulin-Like Growth Factor I (IGF-I) Secretion in Porcine Stromal-Vascular Cultures

Neal X. Chen, G. J. Hausman, J. T. Wright

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

We examined the time course of insulin-like growth factor binding protein (IGFBP) secretion and hormonal regulation of IGFBP and IGF-I secretion in porcine stromal vascular (S-V) cultures. Primary cultures of S-V cells derived from porcine adipose tissue were exposed to serum-free media with or without hormone treatment. Time course studies indicated that secretion of four IGFBP (IGFBP-1, -2, - 3 and -4) by porcine S-V cells increased with time during the first 3 d after the switch to serum-free conditions and then decreased gradually. Growth hormone treatment stimulated secretion of each IGFBP by 47 ± 4.7, 46 ± 10.2, 70 ± 13.2, and 49 ± 5.6%, respectively, over control levels. The secretion of IGFBP-1, -2, -3, and -4 was enhanced by 91 ± 18, 80 ± 16, 74 ± 12, and 263 ± 48%, respectively, in T4-treated S-V cultures compared with untreated cultures. In contrast, dexamethasone reduced the abundance of the IGFBP by 28 to 50% of control levels. Insulin-like growth factor I secretion (844.63 ± 35.98 pg/mL) in vitro (3 d conditioned media) was increased (P < .05) by GH (1,302.45 ± 12.95 pg/mL) and T4 (1,291.60 ± 86.4 pg/mL) and decreased (P < .05) by dexamethasone (552.5 ± 30.2 pg/mL) (n = 4, S-V cell pools, P < .05). In addition to preadipocytes, other cells in S-V cultures also secrete IGFBP. In conclusion, the secretion of IGF-I and IGFBP by S-V cells is differentially regulated by hormones in vitro.

Original languageEnglish (US)
Pages (from-to)2369-2375
Number of pages7
JournalJournal of animal science
Volume74
Issue number10
DOIs
StatePublished - Oct 1996
Externally publishedYes

Keywords

  • Adipocytes
  • Binding Proteins
  • Hormones
  • Insulin-Like Growth Factor I
  • Regulation

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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