Hormone-induced phosphorylation of the plasma membrane calcium pump in cultured aortic endothelial cells

Kevin K W Wang, Yansheng Du, Clement Diglio, Wayne Tsang, Tuan H. Kuo

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The regulation of the plasma membrane Ca2+ pump by hormones via phosphorylation in intact cells has not been clearly established. We now present evidence that the Ca2+ pump is phosphorylated on both serine and threonine residues in unstimulated and stimulated cultured rat aortic endothelial cells. Among the stimuli tested, the protein kinase C activator phorbol 12-myristate 13-acetate (PMA) was most potent and increased the level of phosphorylation threefold, while the cAMP-dependent protein kinase activator 8-(4-chlorophenylthio)-cAMP (CPT-cAMP) stimulated the phosphorylation 1.6-fold. Two-dimensional tryptic phosphopeptide maps of the Ca2+ pump from unstimulated and CPT-cAMP-stimulated cells have identical patterns (five phosphopeptides) while PMA-stimulated cells have three additional phosphopeptides. Isoproterenol-, ATP-, angiotensin II-, and bradykinin-stimulated cells also have increased levels of Ca2+ pump phosphorylation. Stimuli-induced phosphorylation of the Ca2+ pump was rapid (5-10 min) and was concomitant with stimulated calcium efflux from the same cells. This is the first direct evidence that the plasma membrane Ca2+ pump in intact cells is regulated by various hormones or agonists via cAMP-dependent protein kinase or protein kinase C phosphorylation.

Original languageEnglish (US)
Pages (from-to)103-108
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume289
Issue number1
DOIs
StatePublished - Aug 15 1991
Externally publishedYes

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Phosphorylation
Endothelial cells
Cell membranes
Endothelial Cells
Cell Membrane
Pumps
Hormones
Calcium
Phosphopeptides
Cyclic AMP-Dependent Protein Kinases
Protein Kinase C
Acetates
Bradykinin
Threonine
Isoproterenol
Angiotensin II
Serine
Rats
Adenosine Triphosphate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hormone-induced phosphorylation of the plasma membrane calcium pump in cultured aortic endothelial cells. / Wang, Kevin K W; Du, Yansheng; Diglio, Clement; Tsang, Wayne; Kuo, Tuan H.

In: Archives of Biochemistry and Biophysics, Vol. 289, No. 1, 15.08.1991, p. 103-108.

Research output: Contribution to journalArticle

Wang, Kevin K W ; Du, Yansheng ; Diglio, Clement ; Tsang, Wayne ; Kuo, Tuan H. / Hormone-induced phosphorylation of the plasma membrane calcium pump in cultured aortic endothelial cells. In: Archives of Biochemistry and Biophysics. 1991 ; Vol. 289, No. 1. pp. 103-108.
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