HOX genes in human lung: Altered expression in primary pulmonary hypertension and emphysema

Heiko A. Golpon, Mark W. Geraci, Mark D. Moore, Heidi L. Miller, Gary J. Miller, Rubin M. Tuder, Norbert F. Voelkel

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

HOX genes belong to the large family of homeodomain genes that function as transcription factors. Animal studies indicate that they play an essential role in lung development. We investigated the expression pattern of HOX genes in human lung tissue by using microarray and degenerate reverse transcriptase-polymerase chain reaction survey techniques. HOX genes predominantly from the 3′ end of clusters A and B were expressed in normal human adult lung and among them HOXA5 was the most abundant, followed by HOXB2 and HOXB6. In fetal (12 weeks old) and diseased lung specimens (emphysema, primary pulmonary hypertension) additional HOX genes from clusters C and D were expressed. Using in situ hybridization, transcripts for HOXA5 were predominantly found in alveolar septal and epithelial cells, both in normal and diseased lungs. A 2.5-fold increase in HOXA5 mRNA expression was demonstrated by quantitative reverse transcriptase-polymerase chain reaction in primary pulmonary hypertension lung specimens when compared to normal lung tissue. In conclusion, we demonstrate that HOX genes are selectively expressed in the human lung. Differences in the pattern of HOX gene expression exist among fetal, adult, and diseased lung specimens. The altered pattern of HOX gene expression may contribute to the development of pulmonary diseases.

Original languageEnglish (US)
Pages (from-to)955-966
Number of pages12
JournalAmerican Journal of Pathology
Volume158
Issue number3
StatePublished - 2001
Externally publishedYes

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Pulmonary Emphysema
Lung
Genes
Lung Diseases
Reverse Transcriptase Polymerase Chain Reaction
Fetal Diseases
Alveolar Epithelial Cells
Gene Expression
Emphysema
Multigene Family
Familial Primary Pulmonary Hypertension
In Situ Hybridization
Transcription Factors
Messenger RNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Golpon, H. A., Geraci, M. W., Moore, M. D., Miller, H. L., Miller, G. J., Tuder, R. M., & Voelkel, N. F. (2001). HOX genes in human lung: Altered expression in primary pulmonary hypertension and emphysema. American Journal of Pathology, 158(3), 955-966.

HOX genes in human lung : Altered expression in primary pulmonary hypertension and emphysema. / Golpon, Heiko A.; Geraci, Mark W.; Moore, Mark D.; Miller, Heidi L.; Miller, Gary J.; Tuder, Rubin M.; Voelkel, Norbert F.

In: American Journal of Pathology, Vol. 158, No. 3, 2001, p. 955-966.

Research output: Contribution to journalArticle

Golpon, HA, Geraci, MW, Moore, MD, Miller, HL, Miller, GJ, Tuder, RM & Voelkel, NF 2001, 'HOX genes in human lung: Altered expression in primary pulmonary hypertension and emphysema', American Journal of Pathology, vol. 158, no. 3, pp. 955-966.
Golpon HA, Geraci MW, Moore MD, Miller HL, Miller GJ, Tuder RM et al. HOX genes in human lung: Altered expression in primary pulmonary hypertension and emphysema. American Journal of Pathology. 2001;158(3):955-966.
Golpon, Heiko A. ; Geraci, Mark W. ; Moore, Mark D. ; Miller, Heidi L. ; Miller, Gary J. ; Tuder, Rubin M. ; Voelkel, Norbert F. / HOX genes in human lung : Altered expression in primary pulmonary hypertension and emphysema. In: American Journal of Pathology. 2001 ; Vol. 158, No. 3. pp. 955-966.
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