Human CD34+ HLA-DR- bone marrow cells contain progenitor cells capable of self-renewal, multilineage differentiation, and long-term in vitro hematopoiesis

Edward Srour, J. E. Brandt, R. A. Briddell, T. Leemhuis, K. Van Besien, R. Hoffman

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Human bone marrow cells expressing CD34 but not HLA-DR were isolated by immunofluorescence flow cytrometric cell sorting. These cells contained a hematopoietic cell (CFU-B1) capable of producing, in an in vitro semisolid culture system, blast-cell-containing colonies, which possessed the capacity for self-renewal and commitment to multipotential hematopoietic differentiation. In addition, CD34+ HLA-DR- marrow cells contained primitive megakaryocyte progenitor cells, the burst-forming unit-megakaryocyte (BFU-MK). A subset of CD34+ HLA-DR- marrow cells lacking the expression of CD15 and CD71 was obtained by flow cytometric cell sorting and was capable of sustaining in vitro hematopoiesis in suspension culture for up to 8 weeks in the absence of a preestablished adherent marrow cell layer. The combinations of IL-3 + IL-1α and IL-3 + IL-6 sustained proliferation of these cells for 8 weeks, induced maximal cellular expansion, and increased the numbers of assayable progenitor cells. These studies demonstrate that human CD34+ HLA-DR- marrow cells and their subsets contain primitive multipotential hematopoietic cells capable of self-renewal and of differentiation into multiple hematopoietic lineages.

Original languageEnglish
Pages (from-to)287-295
Number of pages9
JournalBlood Cells
Volume17
Issue number2
StatePublished - 1991

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Hematopoiesis
HLA-DR Antigens
Bone Marrow Cells
Stem Cells
Bone Marrow
Interleukin-3
Megakaryocyte Progenitor Cells
In Vitro Techniques
Cell Self Renewal
Megakaryocytes
Interleukin-1
Fluorescent Antibody Technique
Interleukin-6
Suspensions
Cell Proliferation

Keywords

  • Cytokines
  • Hematopoiesis
  • Hematopoietic progenitor cells
  • Long-term marrow culture

ASJC Scopus subject areas

  • Hematology

Cite this

Human CD34+ HLA-DR- bone marrow cells contain progenitor cells capable of self-renewal, multilineage differentiation, and long-term in vitro hematopoiesis. / Srour, Edward; Brandt, J. E.; Briddell, R. A.; Leemhuis, T.; Van Besien, K.; Hoffman, R.

In: Blood Cells, Vol. 17, No. 2, 1991, p. 287-295.

Research output: Contribution to journalArticle

Srour, Edward ; Brandt, J. E. ; Briddell, R. A. ; Leemhuis, T. ; Van Besien, K. ; Hoffman, R. / Human CD34+ HLA-DR- bone marrow cells contain progenitor cells capable of self-renewal, multilineage differentiation, and long-term in vitro hematopoiesis. In: Blood Cells. 1991 ; Vol. 17, No. 2. pp. 287-295.
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