Human endothelial colony forming cells undergo vasculogenesis within biphasic calcium phosphate bone tissue engineering constructs

Sheeny K. Lan Levengood, Michael J. Poellmann, Sherrie G. Clark, David Ingram, Mervin Yoder, Amy J. Wagoner Johnson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

An important consideration in bone regeneration is the need for expedited neovascularization within the defect site. Formation of a vascular network is critical for cell viability and normal function leading to tissue regeneration, but spontaneous angiogenesis is too slow to yield sufficient vessel formation. In this pilot study, human umbilical cord blood (hUCB)-derived endothelial colony forming cells (ECFCs) were evaluated for in vivo vasculogenesis in the macropores of biphasic calcium phosphate (BCP)/bone morphogenetic protein-2 (BMP-2) bone tissue engineering constructs. Constructs were implanted on the abdominal wall of NOD/SCID mice for 4 weeks. This study demonstrated in vivo vasculogenesis by human ECFCs within the macropore space of BCP/BMP-2 constructs. The human ECFC-derived vessels anastomosed with the host vasculature and perfused vessels were visible in the very center of the 5 mm diameter, 2.5 mm tall scaffolds. Additionally, the vessels were evenly distributed throughout the construct. This study suggests that scaffolds containing ECFCs have significant potential for expedited neovascularization in bony defects.

Original languageEnglish
Pages (from-to)4222-4228
Number of pages7
JournalActa Biomaterialia
Volume7
Issue number12
DOIs
StatePublished - Dec 2011

Fingerprint

Bone Morphogenetic Protein 2
Calcium phosphate
Tissue Engineering
Tissue engineering
Scaffolds
Bone
Cells
Bone and Bones
Tissue regeneration
Defects
Blood
Proteins
Inbred NOD Mouse
Bone Regeneration
SCID Mice
Abdominal Wall
Fetal Blood
Blood Vessels
Regeneration
Cell Survival

Keywords

  • Angiogenesis and vasculogenesis
  • Bone
  • Calcium phosphate
  • Endothelial cells
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Human endothelial colony forming cells undergo vasculogenesis within biphasic calcium phosphate bone tissue engineering constructs. / Lan Levengood, Sheeny K.; Poellmann, Michael J.; Clark, Sherrie G.; Ingram, David; Yoder, Mervin; Wagoner Johnson, Amy J.

In: Acta Biomaterialia, Vol. 7, No. 12, 12.2011, p. 4222-4228.

Research output: Contribution to journalArticle

Lan Levengood, Sheeny K. ; Poellmann, Michael J. ; Clark, Sherrie G. ; Ingram, David ; Yoder, Mervin ; Wagoner Johnson, Amy J. / Human endothelial colony forming cells undergo vasculogenesis within biphasic calcium phosphate bone tissue engineering constructs. In: Acta Biomaterialia. 2011 ; Vol. 7, No. 12. pp. 4222-4228.
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