Human Fanconi A cells are susceptible to TRAIL-induced apoptosis

Simona Pigullo, Elisa Ferretti, Marina Lanciotti, Maurizio Bruschi, Giovanni Candiano, Johanna Svahn, Laura Haneline, Carlo Dufour, Vito Pistoia, Anna Corcione

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Tumour necrosis factor (TNF) contributes to the pathogenesis of bone marrow failure in Fanconi anaemia (FA) patients. The sensitivity of haematopoietic cells from FA, complementation group A (FANCA) subjects, who represent the majority of FA patients, to TNF-related apoptosis-inducing ligand (TRAIL) is unknown. The human lymphoblastoid FANCA HSC072 cell line and the genetically corrected counterpart HSC072FANCA-neo were tested for apoptoptic response to TRAIL using flow cytometry and Western blotting. FANCA cells were more sensitive to TRAIL-induced apoptosis than their corrected counterparts, indicating that TRAIL negatively regulates haematopoietic FANCA cell lines. This effect involved poly(ADP-ribose) polymerase-1 cleavage and caspase-8 activation.

Original languageEnglish (US)
Pages (from-to)315-318
Number of pages4
JournalBritish journal of haematology
Volume136
Issue number2
DOIs
StatePublished - Jan 1 2007

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TNF-Related Apoptosis-Inducing Ligand
Fanconi Anemia
Apoptosis
Tumor Necrosis Factor-alpha
Cell Line
Caspase 8
Flow Cytometry
Bone Marrow
Western Blotting
Ligands

Keywords

  • Apoptosis
  • Caspase-8
  • Fanconi type A cells
  • Poly(ADP-ribose) polymerase-1 cleavage
  • Tumour necrosis factor-related apoptosis-inducing ligand

ASJC Scopus subject areas

  • Hematology

Cite this

Pigullo, S., Ferretti, E., Lanciotti, M., Bruschi, M., Candiano, G., Svahn, J., ... Corcione, A. (2007). Human Fanconi A cells are susceptible to TRAIL-induced apoptosis. British journal of haematology, 136(2), 315-318. https://doi.org/10.1111/j.1365-2141.2006.06432.x

Human Fanconi A cells are susceptible to TRAIL-induced apoptosis. / Pigullo, Simona; Ferretti, Elisa; Lanciotti, Marina; Bruschi, Maurizio; Candiano, Giovanni; Svahn, Johanna; Haneline, Laura; Dufour, Carlo; Pistoia, Vito; Corcione, Anna.

In: British journal of haematology, Vol. 136, No. 2, 01.01.2007, p. 315-318.

Research output: Contribution to journalArticle

Pigullo, S, Ferretti, E, Lanciotti, M, Bruschi, M, Candiano, G, Svahn, J, Haneline, L, Dufour, C, Pistoia, V & Corcione, A 2007, 'Human Fanconi A cells are susceptible to TRAIL-induced apoptosis', British journal of haematology, vol. 136, no. 2, pp. 315-318. https://doi.org/10.1111/j.1365-2141.2006.06432.x
Pigullo S, Ferretti E, Lanciotti M, Bruschi M, Candiano G, Svahn J et al. Human Fanconi A cells are susceptible to TRAIL-induced apoptosis. British journal of haematology. 2007 Jan 1;136(2):315-318. https://doi.org/10.1111/j.1365-2141.2006.06432.x
Pigullo, Simona ; Ferretti, Elisa ; Lanciotti, Marina ; Bruschi, Maurizio ; Candiano, Giovanni ; Svahn, Johanna ; Haneline, Laura ; Dufour, Carlo ; Pistoia, Vito ; Corcione, Anna. / Human Fanconi A cells are susceptible to TRAIL-induced apoptosis. In: British journal of haematology. 2007 ; Vol. 136, No. 2. pp. 315-318.
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