Human gamma interferon enhances release from phytohemagglutinin-stimulated T4+ lymphocytes of activities that stimulate colony formation by granulocyte-macrophage, erythroid, and multipotential progenitor cells

W. Piacibello, L. Lu, D. Williams, M. Aglietta, B. Y. Rubin, S. Cooper, M. Wachter, F. Gavosto, Hal Broxmeyer

Research output: Contribution to journalArticle

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Abstract

Human gamma interferon (HuIFNγ) was evaluated for its effects on the release from human peripheral blood T lymphocytes (>98% pure) stimulated by phytohemagglutinin (PHA) of activities that can stimulate granolocyte-macrophage (CFU-GM) colonies and clusters, erythroid (BFU-E) bursts, and mixed (CFU-GEMM) colonies. T lymphocytes did not release these activities in the absence of PHA with or without HuIFNγ. In the presence of PHA, pure natural HuIFNγ at concentrations of 0.1 to 100 U/mL significantly enhanced release of these colony-stimulating activities. Although enhanced release of granulocyte-macrophage colony-stimulating activities were noted when T lymphocytes were added to the conditioning medium in the presence of 0.1%, 0.5%, and 1% PHA, enhanced release of burst-promoting and mixed colony activities was seen only in the presence of 0.1% and 0.5% PHA. The enhanced release of colony-stimulating activities was not due to HuIFNγ-suppression of the release from PHA-stimulated T lymphocytes of suppressor molecules. The enhancing effects of natural HuIFNγ were neutralized with a monoclonal anti-natural HuIFNγ, and recombinant HuIFNγ mimicked the enhancing effects of the natural HuIFNγ. This enhancing effect was noted only when HuIFNγ was added with the T lymphocytes and PHA during the first 24 hours of incubation. T lymphocytes were separated into T4+, T8-, T8+, and T4- subsets (>98% pure for the appropriate phenotypes) after incubation with OKT4- and OKT8- monoclonal antibodies and sorting on a fluorescence-activated cell sorter (FACS). All types of colony-stimulating activities were released from each population after stimulation with PHA, but enhanced release of these activities in the presence of HuIFNγ was only detected with the T4+ or T8- subsets of lymphocytes. It cannot be concluded from these studies whether HuIFNγ is enhancing the release of one or several types of colony-stimulating activities, but these studies suggest a role for HuIFNγ and T4+ lymphocyte subsets in the regulation in vitro of the release of colony-stimulating activities.

Original languageEnglish (US)
Pages (from-to)1339-1347
Number of pages9
JournalBlood
Volume68
Issue number6
StatePublished - 1986
Externally publishedYes

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CD4-Positive T-Lymphocytes
Erythroid Precursor Cells
Lymphocytes
Macrophages
Phytohemagglutinins
Granulocytes
T-cells
T-Lymphocytes
human IFNG protein
CD8-Positive T-Lymphocytes
Myeloid Progenitor Cells
Granulocyte-Macrophage Progenitor Cells
Lymphocyte Subsets
Sorting

ASJC Scopus subject areas

  • Hematology

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Human gamma interferon enhances release from phytohemagglutinin-stimulated T4+ lymphocytes of activities that stimulate colony formation by granulocyte-macrophage, erythroid, and multipotential progenitor cells. / Piacibello, W.; Lu, L.; Williams, D.; Aglietta, M.; Rubin, B. Y.; Cooper, S.; Wachter, M.; Gavosto, F.; Broxmeyer, Hal.

In: Blood, Vol. 68, No. 6, 1986, p. 1339-1347.

Research output: Contribution to journalArticle

Piacibello, W. ; Lu, L. ; Williams, D. ; Aglietta, M. ; Rubin, B. Y. ; Cooper, S. ; Wachter, M. ; Gavosto, F. ; Broxmeyer, Hal. / Human gamma interferon enhances release from phytohemagglutinin-stimulated T4+ lymphocytes of activities that stimulate colony formation by granulocyte-macrophage, erythroid, and multipotential progenitor cells. In: Blood. 1986 ; Vol. 68, No. 6. pp. 1339-1347.
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abstract = "Human gamma interferon (HuIFNγ) was evaluated for its effects on the release from human peripheral blood T lymphocytes (>98{\%} pure) stimulated by phytohemagglutinin (PHA) of activities that can stimulate granolocyte-macrophage (CFU-GM) colonies and clusters, erythroid (BFU-E) bursts, and mixed (CFU-GEMM) colonies. T lymphocytes did not release these activities in the absence of PHA with or without HuIFNγ. In the presence of PHA, pure natural HuIFNγ at concentrations of 0.1 to 100 U/mL significantly enhanced release of these colony-stimulating activities. Although enhanced release of granulocyte-macrophage colony-stimulating activities were noted when T lymphocytes were added to the conditioning medium in the presence of 0.1{\%}, 0.5{\%}, and 1{\%} PHA, enhanced release of burst-promoting and mixed colony activities was seen only in the presence of 0.1{\%} and 0.5{\%} PHA. The enhanced release of colony-stimulating activities was not due to HuIFNγ-suppression of the release from PHA-stimulated T lymphocytes of suppressor molecules. The enhancing effects of natural HuIFNγ were neutralized with a monoclonal anti-natural HuIFNγ, and recombinant HuIFNγ mimicked the enhancing effects of the natural HuIFNγ. This enhancing effect was noted only when HuIFNγ was added with the T lymphocytes and PHA during the first 24 hours of incubation. T lymphocytes were separated into T4+, T8-, T8+, and T4- subsets (>98{\%} pure for the appropriate phenotypes) after incubation with OKT4- and OKT8- monoclonal antibodies and sorting on a fluorescence-activated cell sorter (FACS). All types of colony-stimulating activities were released from each population after stimulation with PHA, but enhanced release of these activities in the presence of HuIFNγ was only detected with the T4+ or T8- subsets of lymphocytes. It cannot be concluded from these studies whether HuIFNγ is enhancing the release of one or several types of colony-stimulating activities, but these studies suggest a role for HuIFNγ and T4+ lymphocyte subsets in the regulation in vitro of the release of colony-stimulating activities.",
author = "W. Piacibello and L. Lu and D. Williams and M. Aglietta and Rubin, {B. Y.} and S. Cooper and M. Wachter and F. Gavosto and Hal Broxmeyer",
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T1 - Human gamma interferon enhances release from phytohemagglutinin-stimulated T4+ lymphocytes of activities that stimulate colony formation by granulocyte-macrophage, erythroid, and multipotential progenitor cells

AU - Piacibello, W.

AU - Lu, L.

AU - Williams, D.

AU - Aglietta, M.

AU - Rubin, B. Y.

AU - Cooper, S.

AU - Wachter, M.

AU - Gavosto, F.

AU - Broxmeyer, Hal

PY - 1986

Y1 - 1986

N2 - Human gamma interferon (HuIFNγ) was evaluated for its effects on the release from human peripheral blood T lymphocytes (>98% pure) stimulated by phytohemagglutinin (PHA) of activities that can stimulate granolocyte-macrophage (CFU-GM) colonies and clusters, erythroid (BFU-E) bursts, and mixed (CFU-GEMM) colonies. T lymphocytes did not release these activities in the absence of PHA with or without HuIFNγ. In the presence of PHA, pure natural HuIFNγ at concentrations of 0.1 to 100 U/mL significantly enhanced release of these colony-stimulating activities. Although enhanced release of granulocyte-macrophage colony-stimulating activities were noted when T lymphocytes were added to the conditioning medium in the presence of 0.1%, 0.5%, and 1% PHA, enhanced release of burst-promoting and mixed colony activities was seen only in the presence of 0.1% and 0.5% PHA. The enhanced release of colony-stimulating activities was not due to HuIFNγ-suppression of the release from PHA-stimulated T lymphocytes of suppressor molecules. The enhancing effects of natural HuIFNγ were neutralized with a monoclonal anti-natural HuIFNγ, and recombinant HuIFNγ mimicked the enhancing effects of the natural HuIFNγ. This enhancing effect was noted only when HuIFNγ was added with the T lymphocytes and PHA during the first 24 hours of incubation. T lymphocytes were separated into T4+, T8-, T8+, and T4- subsets (>98% pure for the appropriate phenotypes) after incubation with OKT4- and OKT8- monoclonal antibodies and sorting on a fluorescence-activated cell sorter (FACS). All types of colony-stimulating activities were released from each population after stimulation with PHA, but enhanced release of these activities in the presence of HuIFNγ was only detected with the T4+ or T8- subsets of lymphocytes. It cannot be concluded from these studies whether HuIFNγ is enhancing the release of one or several types of colony-stimulating activities, but these studies suggest a role for HuIFNγ and T4+ lymphocyte subsets in the regulation in vitro of the release of colony-stimulating activities.

AB - Human gamma interferon (HuIFNγ) was evaluated for its effects on the release from human peripheral blood T lymphocytes (>98% pure) stimulated by phytohemagglutinin (PHA) of activities that can stimulate granolocyte-macrophage (CFU-GM) colonies and clusters, erythroid (BFU-E) bursts, and mixed (CFU-GEMM) colonies. T lymphocytes did not release these activities in the absence of PHA with or without HuIFNγ. In the presence of PHA, pure natural HuIFNγ at concentrations of 0.1 to 100 U/mL significantly enhanced release of these colony-stimulating activities. Although enhanced release of granulocyte-macrophage colony-stimulating activities were noted when T lymphocytes were added to the conditioning medium in the presence of 0.1%, 0.5%, and 1% PHA, enhanced release of burst-promoting and mixed colony activities was seen only in the presence of 0.1% and 0.5% PHA. The enhanced release of colony-stimulating activities was not due to HuIFNγ-suppression of the release from PHA-stimulated T lymphocytes of suppressor molecules. The enhancing effects of natural HuIFNγ were neutralized with a monoclonal anti-natural HuIFNγ, and recombinant HuIFNγ mimicked the enhancing effects of the natural HuIFNγ. This enhancing effect was noted only when HuIFNγ was added with the T lymphocytes and PHA during the first 24 hours of incubation. T lymphocytes were separated into T4+, T8-, T8+, and T4- subsets (>98% pure for the appropriate phenotypes) after incubation with OKT4- and OKT8- monoclonal antibodies and sorting on a fluorescence-activated cell sorter (FACS). All types of colony-stimulating activities were released from each population after stimulation with PHA, but enhanced release of these activities in the presence of HuIFNγ was only detected with the T4+ or T8- subsets of lymphocytes. It cannot be concluded from these studies whether HuIFNγ is enhancing the release of one or several types of colony-stimulating activities, but these studies suggest a role for HuIFNγ and T4+ lymphocyte subsets in the regulation in vitro of the release of colony-stimulating activities.

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