Human immune response to HIV-1 Nef. II. Induction of HIV-1/HIV-2 Nef cross-reactive cytotoxic T lymphocytes in peripheral blood lymphocytes of non-infected healthy individuals

Maria Lucchiarr-Hartz, Monika Bauer, Gabriele Niedermann, Bernhard Maier, Andreas Meyerhans, Klaus Eichmann

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

HIV-specific CD8+ cytotoxic T lymphocytes (CTL) are thought to have a beneficial role in HIV infection. In a previous report we have shown that HIV-1 Nef-specific CTL can be readily induced in peripheral blood lymphocytes of seronegative healthy young adults by in vitro stimulation with autologous Epstein-Barr virus-transformed B lymphoblastoid cell lines transfected with the HIV-1 nef gene. Here we demonstrate that these Nef-specific CTL can efficiently lyse HIV-infected primary CD4+ T lymphocytes. CTL of the blood donor tested were Nef-specific and restricted by the autologous MHC class I molecules HLA-A2 and HLA-B7. They recognized HIV-1 Nef in association with both restriction elements but HIV-2 Nef only in association with HLA-B7. The cross-reactivity of the induced effector cells together with the potent immunogenicity of Nef in healthy seronegatives further support the inclusion of Nef as a constituent of HIV vaccines.

Original languageEnglish (US)
Pages (from-to)577-584
Number of pages8
JournalInternational immunology
Volume8
Issue number4
DOIs
StatePublished - 1996

Keywords

  • Cytotoxic T lymphocytes
  • HIV Nef
  • HIV-1
  • HIV-2
  • Human

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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