Human insulin-related DNA sequences map to chromosomes 2 and 11

Peter Rotwein, Susan L. Naylor, John Chirgwin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The insulin-related hormone family consists of four known peptides: insulin, the insulin-like growth factors I and II, and relaxin. To investigate the hypothesis that the family contains additional members, we have isolated a series of human genomic clones using the insulin gene as a hybridization probe. Two of these single copy DNA sequences, hIr 1 and hIr 2, have been localized to chromosome 2 and 11p11→q13, respectively, by Southern blot analysis of mouse-human somatic cell hybrids, a result consistent with other evidence supporting the dispersal of the insulin gene family throughout the genome. Although a biological function for these DNA sequences has not yet been established, hIr1 and hIr2 are potentially useful as molecular probes for mapping, by analysis of restriction fragment length polymorphisms (RFLP), genetic disorders linked to chromosomes 2 or 11.

Original languageEnglish (US)
Pages (from-to)625-631
Number of pages7
JournalSomatic Cell and Molecular Genetics
Volume12
Issue number6
DOIs
StatePublished - Nov 1986
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 2
Insulin
Relaxin
Molecular Probes
Inborn Genetic Diseases
Insulin-Like Growth Factor II
Hybrid Cells
Southern Blotting
Insulin-Like Growth Factor I
Restriction Fragment Length Polymorphisms
Genes
Clone Cells
Genome
Hormones
Peptides

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Human insulin-related DNA sequences map to chromosomes 2 and 11. / Rotwein, Peter; Naylor, Susan L.; Chirgwin, John.

In: Somatic Cell and Molecular Genetics, Vol. 12, No. 6, 11.1986, p. 625-631.

Research output: Contribution to journalArticle

Rotwein, Peter ; Naylor, Susan L. ; Chirgwin, John. / Human insulin-related DNA sequences map to chromosomes 2 and 11. In: Somatic Cell and Molecular Genetics. 1986 ; Vol. 12, No. 6. pp. 625-631.
@article{ada9d4457c2b44328a4093d92bd343ff,
title = "Human insulin-related DNA sequences map to chromosomes 2 and 11",
abstract = "The insulin-related hormone family consists of four known peptides: insulin, the insulin-like growth factors I and II, and relaxin. To investigate the hypothesis that the family contains additional members, we have isolated a series of human genomic clones using the insulin gene as a hybridization probe. Two of these single copy DNA sequences, hIr 1 and hIr 2, have been localized to chromosome 2 and 11p11→q13, respectively, by Southern blot analysis of mouse-human somatic cell hybrids, a result consistent with other evidence supporting the dispersal of the insulin gene family throughout the genome. Although a biological function for these DNA sequences has not yet been established, hIr1 and hIr2 are potentially useful as molecular probes for mapping, by analysis of restriction fragment length polymorphisms (RFLP), genetic disorders linked to chromosomes 2 or 11.",
author = "Peter Rotwein and Naylor, {Susan L.} and John Chirgwin",
year = "1986",
month = "11",
doi = "10.1007/BF01671947",
language = "English (US)",
volume = "12",
pages = "625--631",
journal = "Somatic Cell and Molecular Genetics",
issn = "0740-7750",
publisher = "Springer GmbH & Co, Auslieferungs-Gesellschaf",
number = "6",

}

TY - JOUR

T1 - Human insulin-related DNA sequences map to chromosomes 2 and 11

AU - Rotwein, Peter

AU - Naylor, Susan L.

AU - Chirgwin, John

PY - 1986/11

Y1 - 1986/11

N2 - The insulin-related hormone family consists of four known peptides: insulin, the insulin-like growth factors I and II, and relaxin. To investigate the hypothesis that the family contains additional members, we have isolated a series of human genomic clones using the insulin gene as a hybridization probe. Two of these single copy DNA sequences, hIr 1 and hIr 2, have been localized to chromosome 2 and 11p11→q13, respectively, by Southern blot analysis of mouse-human somatic cell hybrids, a result consistent with other evidence supporting the dispersal of the insulin gene family throughout the genome. Although a biological function for these DNA sequences has not yet been established, hIr1 and hIr2 are potentially useful as molecular probes for mapping, by analysis of restriction fragment length polymorphisms (RFLP), genetic disorders linked to chromosomes 2 or 11.

AB - The insulin-related hormone family consists of four known peptides: insulin, the insulin-like growth factors I and II, and relaxin. To investigate the hypothesis that the family contains additional members, we have isolated a series of human genomic clones using the insulin gene as a hybridization probe. Two of these single copy DNA sequences, hIr 1 and hIr 2, have been localized to chromosome 2 and 11p11→q13, respectively, by Southern blot analysis of mouse-human somatic cell hybrids, a result consistent with other evidence supporting the dispersal of the insulin gene family throughout the genome. Although a biological function for these DNA sequences has not yet been established, hIr1 and hIr2 are potentially useful as molecular probes for mapping, by analysis of restriction fragment length polymorphisms (RFLP), genetic disorders linked to chromosomes 2 or 11.

UR - http://www.scopus.com/inward/record.url?scp=0022978966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022978966&partnerID=8YFLogxK

U2 - 10.1007/BF01671947

DO - 10.1007/BF01671947

M3 - Article

C2 - 3024335

AN - SCOPUS:0022978966

VL - 12

SP - 625

EP - 631

JO - Somatic Cell and Molecular Genetics

JF - Somatic Cell and Molecular Genetics

SN - 0740-7750

IS - 6

ER -