Human lung project

Evaluating variance of gene expression in the human lung

Michael P. Gruber, Christopher D. Coldren, Malcolm D. Woolum, Gregory P. Cosgrove, Chan Zeng, Anna E. Barón, Mark D. Moore, Carlyne D. Cool, G. Scott Worthen, Kevin K. Brown, Mark W. Geraci

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Nondiseased tissue is an important reference for microarray studies of pulmonary disease. We obtained 23 single lungs from multiorgan donors at time of procurement. Donors varied in age, sex, smoking history, and ethnicity. Lungs were dissected into upper and lower lobe peripheral sections for RNA extraction. Microarray analysis was performed using Affymetrix Hu-133 Plus 2.0 arrays. We observed that the relative variability of gene expression increased rapidly from technical (lowest), to regional, to population (highest). In addition, age and sex have measurable effects on gene expression. Gene expression variability is heterogeneously distributed among biologic categories. We conclude that gene expression variability is greater between individuals than within individuals and that population variability is the most important factor in the study design of microarray experiments of the human lung. Classes of genes with high population variability are biologically important and provide a novel perspective into lung physiology and pathobiology. Our study represents the first comprehensive analysis of non-diseased lung tissue. The generation of this robust dataset has important implications for the design and implementation of future comparative expression analysis with pulmonary disease states.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume35
Issue number1
DOIs
StatePublished - Jul 2006
Externally publishedYes

Fingerprint

Gene expression
Microarrays
Gene Expression
Lung
Pulmonary diseases
Lung Diseases
Tissue
Physiology
Population
Microarray Analysis
Genes
RNA
Smoking
History
Experiments

Keywords

  • Genomics
  • Lung
  • Microarray
  • Variability

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Gruber, M. P., Coldren, C. D., Woolum, M. D., Cosgrove, G. P., Zeng, C., Barón, A. E., ... Geraci, M. W. (2006). Human lung project: Evaluating variance of gene expression in the human lung. American Journal of Respiratory Cell and Molecular Biology, 35(1), 65-71. https://doi.org/10.1165/rcmb.2004-0261OC

Human lung project : Evaluating variance of gene expression in the human lung. / Gruber, Michael P.; Coldren, Christopher D.; Woolum, Malcolm D.; Cosgrove, Gregory P.; Zeng, Chan; Barón, Anna E.; Moore, Mark D.; Cool, Carlyne D.; Worthen, G. Scott; Brown, Kevin K.; Geraci, Mark W.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 35, No. 1, 07.2006, p. 65-71.

Research output: Contribution to journalArticle

Gruber, MP, Coldren, CD, Woolum, MD, Cosgrove, GP, Zeng, C, Barón, AE, Moore, MD, Cool, CD, Worthen, GS, Brown, KK & Geraci, MW 2006, 'Human lung project: Evaluating variance of gene expression in the human lung', American Journal of Respiratory Cell and Molecular Biology, vol. 35, no. 1, pp. 65-71. https://doi.org/10.1165/rcmb.2004-0261OC
Gruber, Michael P. ; Coldren, Christopher D. ; Woolum, Malcolm D. ; Cosgrove, Gregory P. ; Zeng, Chan ; Barón, Anna E. ; Moore, Mark D. ; Cool, Carlyne D. ; Worthen, G. Scott ; Brown, Kevin K. ; Geraci, Mark W. / Human lung project : Evaluating variance of gene expression in the human lung. In: American Journal of Respiratory Cell and Molecular Biology. 2006 ; Vol. 35, No. 1. pp. 65-71.
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