Human multipotential progenitor cells (CFU-GEMM) have extensive replating capacity for secondary CFU-GEMM: An effect enhanced by cord blood plasma

Catherine E. Carow, Giao Hangoc, Hal Broxmeyer

Research output: Contribution to journalArticle

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Abstract

The replating capability of human umbilical cord blood (CB) multipotential (CFU-GEMM) progenitors was assessed in vitro as an estimate of self-renewal using erythropoietin (Epo), steel factor (SLF), and either fetal bovine serum (FBS) or CB plasma. This study found a much higher replating efficiency for CB CFU-GEMM than previously reported, in terms of the percentage of colonies that could be replated, the number of secondary colonies per replated primary colony, and the size of secondary colonies. Moreover, the majority of secondary colonies were CFU-GEMM-derived. Although the percentages of bone marrow CFU-GEMM that replate was similar to that for CB CFU-GEMM and the sizes of secondary bone marrow and CB CFU-GEMM were also similar, replated CB CFU-GEMM gave rise to far greater numbers of secondary colonies. No tertiary colonies were observed when secondary CFU-GEMM were replated. Detection of extensive secondary replating potential was enhanced by the addition of CB plasma to the cultures. This activity was not found in either adult blood (PB) plasma, umbilical cord vein endothelial cell-conditioned medium (ECCM), FBS plus ECCM, or FBS plus the combination of interleukin-1 (IL-1), IL-3, IL-6, IL-11, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor. Whether the CB plasma-enhancing activity for CFU-GEMM replating capacity is attributable to a novel factor or factors, or represents effects of other known cytokines, alone or in combination, remains to be determined. Of particular relevance, these studies suggest that human CFU-GEMM have some degree of stemness and perhaps should be classified as a subset of stem cells.

Original languageEnglish
Pages (from-to)942-949
Number of pages8
JournalBlood
Volume81
Issue number4
StatePublished - Feb 15 1993

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Myeloid Progenitor Cells
Fetal Blood
Blood
Stem Cells
Plasmas
Endothelial cells
Conditioned Culture Medium
Bone
Endothelial Cells
Interleukin-11
Bone Marrow
Serum
Stem Cell Factor
Interleukin-3
Umbilical Veins
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Erythropoietin
Umbilical Cord
Stem cells

ASJC Scopus subject areas

  • Hematology

Cite this

Human multipotential progenitor cells (CFU-GEMM) have extensive replating capacity for secondary CFU-GEMM : An effect enhanced by cord blood plasma. / Carow, Catherine E.; Hangoc, Giao; Broxmeyer, Hal.

In: Blood, Vol. 81, No. 4, 15.02.1993, p. 942-949.

Research output: Contribution to journalArticle

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