Human natural killer cell adhesion molecules

Differential expression after activation and participation in cytolysis

Michael Robertson, Michael A. Caligiuri, Thomas J. Manley, Herbert Levine, Jerome Ritz

Research output: Contribution to journalArticle

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Abstract

Cell adhesion molecules (CAM) participate in interactions between lymphocytes, accessory cells, and target cells that are critical in the generation of effective immune responses. To characterize the involvement of CAM in NK and lymphokine activated killer (LAK) activities, we examined the expression of several CAM by freshly isolated human NK cells and by NK cells activated in vitro with IL-2, and compared this to CAM expression by T lymphocytes under similar conditions. Freshly isolated human NK cells were uniformly LFA-3 (CD58)+ and expressed two to three-fold higher surface levels of LFA-1 (CD11a/CD18) than resting T lymphocytes. More NK cells than T cells also expressed phenotypically detectable levels of intercellular adhesion molecule-1 (CD54). After in vitro incubation with IL-2, human NK cells demonstrated four-to sixfold increases in surface levels of CD11a/CD18, CD2, CD54, CD58, and the NK cell-associated Ag NKH-1 (CD56). Furthermore, essentially all NK cells became CD54+ within 3 days of exposure to IL-2. T cells did not demonstrate comparable up-regulation of CAM after incubation with IL-2. Increases in NK cell CAM expression were associated with enhanced formation of E:T cell conjugates, enhanced killing of NK-sensitive targets, and the induction of cytotoxicity for previously NK-resistant targets (LAK activity). The LAK activity induced by exogenous IL-2 could be partially inhibited by anti-CD2, anti-CD11a, or anti-CD54 antibodies and almost completely abrogated by anti-CD2 and anti-CD11a in combination. These studies suggest that CAM play a central role in the regulation of NK cytolysis, and that changes in CAM expression may alter the target cell specificity of activated NK effectors.

Original languageEnglish (US)
Pages (from-to)3194-3201
Number of pages8
JournalJournal of Immunology
Volume145
Issue number10
StatePublished - Nov 15 1990
Externally publishedYes

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Cell Adhesion Molecules
Natural Killer Cells
Interleukin-2
Lymphokines
T-Lymphocytes
CD58 Antigens
Lymphocyte Function-Associated Antigen-1
Intercellular Adhesion Molecule-1
Anti-Idiotypic Antibodies
Up-Regulation
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Human natural killer cell adhesion molecules : Differential expression after activation and participation in cytolysis. / Robertson, Michael; Caligiuri, Michael A.; Manley, Thomas J.; Levine, Herbert; Ritz, Jerome.

In: Journal of Immunology, Vol. 145, No. 10, 15.11.1990, p. 3194-3201.

Research output: Contribution to journalArticle

Robertson, Michael ; Caligiuri, Michael A. ; Manley, Thomas J. ; Levine, Herbert ; Ritz, Jerome. / Human natural killer cell adhesion molecules : Differential expression after activation and participation in cytolysis. In: Journal of Immunology. 1990 ; Vol. 145, No. 10. pp. 3194-3201.
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