Human papillomavirus type 11 (HPV-11) neutralizing antibodies in the serum and genital mucosal secretions of African green monkeys immunized with HPV-11 virus-like particles expressed in yeast

Robert S. Lowe, Darron Brown, Janine T. Bryan, James C. Cook, Hugh A. George, Kathryn J. Hofmann, William M. Hurni, Joseph G. Joyce, E. Dale Lehman, Henry Z. Markus, Michael P. Neeper, Loren D. Schultz, Alan R. Shaw, Kathrin U. Jansen

Research output: Contribution to journalArticle

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Abstract

It has been shown previously that immunization of animals with recombinant virus-like particles (VLPs) consisting of the viral capsid proteins L1 or L1 plus L2 protected animals against experimental vital challenge. However, none of these experimental models addresses the issue of whether systemic immunization with VLPs elicits a neutralizing antibody response in the genital mucosa. Such a response may be necessary to protect the uterine cervix against infection with genital human papillomavirus (HPV) types. African green monkeys systemically immunized with HPV-11 VLPs expressed in Saccharomyces cerevisiae and formulated on aluminum adjuvant elicited high-titered HPV-11 VLP-specific serum antibody responses. Sera from these immunized monkeys neutralized HPV-11 in the athymic mouse xenograft system. Significant levels of HPV-11-neutralizing antibodies also were observed in cervicovaginal secretions. These findings suggest that protection against HPV infection of the uterine cervix may be possible through systemic immunization with HPV VLPs.

Original languageEnglish (US)
Pages (from-to)1141-1145
Number of pages5
JournalJournal of Infectious Diseases
Volume176
Issue number5
StatePublished - 1997
Externally publishedYes

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Papillomaviridae
Human papillomavirus 11
Cercopithecus aethiops
Neutralizing Antibodies
Virion
Yeasts
Immunization
Serum
Cervix Uteri
Antibody Formation
Papillomavirus Infections
Capsid Proteins
Viral Proteins
Aluminum
Heterografts
Nude Mice
Haplorhini
Saccharomyces cerevisiae
Mucous Membrane
Theoretical Models

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Human papillomavirus type 11 (HPV-11) neutralizing antibodies in the serum and genital mucosal secretions of African green monkeys immunized with HPV-11 virus-like particles expressed in yeast. / Lowe, Robert S.; Brown, Darron; Bryan, Janine T.; Cook, James C.; George, Hugh A.; Hofmann, Kathryn J.; Hurni, William M.; Joyce, Joseph G.; Dale Lehman, E.; Markus, Henry Z.; Neeper, Michael P.; Schultz, Loren D.; Shaw, Alan R.; Jansen, Kathrin U.

In: Journal of Infectious Diseases, Vol. 176, No. 5, 1997, p. 1141-1145.

Research output: Contribution to journalArticle

Lowe, RS, Brown, D, Bryan, JT, Cook, JC, George, HA, Hofmann, KJ, Hurni, WM, Joyce, JG, Dale Lehman, E, Markus, HZ, Neeper, MP, Schultz, LD, Shaw, AR & Jansen, KU 1997, 'Human papillomavirus type 11 (HPV-11) neutralizing antibodies in the serum and genital mucosal secretions of African green monkeys immunized with HPV-11 virus-like particles expressed in yeast', Journal of Infectious Diseases, vol. 176, no. 5, pp. 1141-1145.
Lowe, Robert S. ; Brown, Darron ; Bryan, Janine T. ; Cook, James C. ; George, Hugh A. ; Hofmann, Kathryn J. ; Hurni, William M. ; Joyce, Joseph G. ; Dale Lehman, E. ; Markus, Henry Z. ; Neeper, Michael P. ; Schultz, Loren D. ; Shaw, Alan R. ; Jansen, Kathrin U. / Human papillomavirus type 11 (HPV-11) neutralizing antibodies in the serum and genital mucosal secretions of African green monkeys immunized with HPV-11 virus-like particles expressed in yeast. In: Journal of Infectious Diseases. 1997 ; Vol. 176, No. 5. pp. 1141-1145.
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