Human papillomavirus type 11 (HPV-11) neutralizing antibodies in the serum and genital mucosal secretions of African green monkeys immunized with HPV-11 virus-like particles expressed in yeast

Robert S. Lowe, Darron R. Brown, Janine T. Bryan, James C. Cook, Hugh A. George, Kathryn J. Hofmann, William M. Hurni, Joseph G. Joyce, E. Dale Lehman, Henry Z. Markus, Michael P. Neeper, Loren D. Schultz, Alan R. Shaw, Kathrin U. Jansen

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

It has been shown previously that immunization of animals with recombinant virus-like particles (VLPs) consisting of the viral capsid proteins L1 or L1 plus L2 protected animals against experimental vital challenge. However, none of these experimental models addresses the issue of whether systemic immunization with VLPs elicits a neutralizing antibody response in the genital mucosa. Such a response may be necessary to protect the uterine cervix against infection with genital human papillomavirus (HPV) types. African green monkeys systemically immunized with HPV-11 VLPs expressed in Saccharomyces cerevisiae and formulated on aluminum adjuvant elicited high-titered HPV-11 VLP-specific serum antibody responses. Sera from these immunized monkeys neutralized HPV-11 in the athymic mouse xenograft system. Significant levels of HPV-11-neutralizing antibodies also were observed in cervicovaginal secretions. These findings suggest that protection against HPV infection of the uterine cervix may be possible through systemic immunization with HPV VLPs.

Original languageEnglish (US)
Pages (from-to)1141-1145
Number of pages5
JournalJournal of Infectious Diseases
Volume176
Issue number5
DOIs
StatePublished - Jan 1 1997

    Fingerprint

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Lowe, R. S., Brown, D. R., Bryan, J. T., Cook, J. C., George, H. A., Hofmann, K. J., Hurni, W. M., Joyce, J. G., Dale Lehman, E., Markus, H. Z., Neeper, M. P., Schultz, L. D., Shaw, A. R., & Jansen, K. U. (1997). Human papillomavirus type 11 (HPV-11) neutralizing antibodies in the serum and genital mucosal secretions of African green monkeys immunized with HPV-11 virus-like particles expressed in yeast. Journal of Infectious Diseases, 176(5), 1141-1145. https://doi.org/10.1086/514105