Human papillomavirus type 11 neutralization in the athymic mouse xenograft system: Correlation with virus-like particle IgG concentration

Janine T. Bryan, Kathrin U. Jansen, Robert S. Lowe, Kenneth H. Fife, Tracy McClowry, Dessa Glass, Darron R. Brown

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Neutralization of virus is likely to be necessary for development of an effective prophylactic vaccine against genital human papillomavirus (HPV) infection. Two New Zealand white rabbits were immunized with purified HPV type 11 (HPV 11) virions in Freund's adjuvant. An enzyme linked immunoassay (ELISA) was used to determine the quantity of IgG which recognized the HPV 11 major capsid protein (L1 protein) virus-like particles (VLPs) in the two anti-HPV 11 sera (serum A and serum B). The concentration of HPV 11 L1 VLP- specific IgG in the A and B sera were determined to be 37 and 90 υg per ml, respectively. The A and B sera were used in neutralization experiments in the athymic mouse xenograft system with known quantities of purified HPV 11 virions. The concentration of HPV 11 L1 VLP-specific IgG required to neutralize HPV 11 was determined for each antiserum. This concentration of IgG was approximately 700 to 900 ng per ml. This study demonstrates a positive correlation between the level of HPV 11 L1 VLP-specific IgG in animals immunized with HPV 11 virions and neutralization of HPV 11 in the athymic mouse model. Further studies are needed 1) to determine if sera or genital secretions from other species are neutralizing in the athymic mouse xenograft system, and 2) to determine if the VLP ELISA can be used as a reliable substitute for more cumbersome neutralization assays.

Original languageEnglish (US)
Pages (from-to)185-188
Number of pages4
JournalJournal of Medical Virology
Volume53
Issue number3
DOIs
StatePublished - Nov 1997

Keywords

  • Anti-body neutralization
  • Athymic mice
  • Human papilloma-virus

ASJC Scopus subject areas

  • Virology

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