Human papillomavirus type 6: Classification of clinical isolates and functional analysis of E2 proteins

Robert Kovelman, Graham K. Bilter, Ann Roman, Darron Brown, Miguel S. Barbosa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Human papillomaviruses (HPVs) cause a variety of clinical manifestations including the most prevalent viral sexually transmitted disease, genital warts. HPV-6 is found in a greater number of genital warts than any other HPV. To increase our understanding of the structural and functional relationships between HPV-6 isolates and to provide information for epidemiological studies, the sequences of the E2, E6 and E7 coding regions of HPV-6 genomes in clinical samples were determined. This sequence analysis was performed on isolates originally designated HPV-6a on the basis of analysis of patterns generated by restriction enzyme digestion. It was found that the designation of subtype on the basis of restriction enzyme digestion correlated poorly with the designation of subtype on the basis of sequence comparison; in fact, the clinical isolates were clearly categorized into HPV-6a and HPV-6b groups, with the previously described HPV-6vc being a member of the HPV-6a group. It was also found that the HPV-6a E2 protein is a much less potent activator of transcription than the HPV-16 E2 protein, generalizing our previous results with the HPV-6b E2 protein to this second HPV-6 E2 protein. These studies indicate that the amino acid differences observed between these natural variants of the HPV-6 E2 protein do not affect its function.

Original languageEnglish
Pages (from-to)2445-2451
Number of pages7
JournalJournal of General Virology
Volume80
Issue number9
StatePublished - 1999

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Human papillomavirus 6
Proteins
Condylomata Acuminata
Viral Sexually Transmitted Diseases
Digestion
Enzymes
Sequence Analysis
Epidemiologic Studies
Genome
Amino Acids
oncogene protein E2, Human papillomavirus type 1

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Human papillomavirus type 6 : Classification of clinical isolates and functional analysis of E2 proteins. / Kovelman, Robert; Bilter, Graham K.; Roman, Ann; Brown, Darron; Barbosa, Miguel S.

In: Journal of General Virology, Vol. 80, No. 9, 1999, p. 2445-2451.

Research output: Contribution to journalArticle

Kovelman, Robert ; Bilter, Graham K. ; Roman, Ann ; Brown, Darron ; Barbosa, Miguel S. / Human papillomavirus type 6 : Classification of clinical isolates and functional analysis of E2 proteins. In: Journal of General Virology. 1999 ; Vol. 80, No. 9. pp. 2445-2451.
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